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. 1974 Oct;10(4):802–809. doi: 10.1128/iai.10.4.802-809.1974

Cellular Immune Activity of a Galactomannan-Protein Complex from Mycelia of Histoplasma capsulatum

E Reiss a,1, W O Mitchell a, S H Stone a, H F Hasenclever a
PMCID: PMC423025  PMID: 4214782

Abstract

A galactomannan-protein complex was extracted from defatted mycelium of Histoplasma capsulatum with 0.25 N alkali at 25 C. It accounted for 7.5% of the cell dry weight in nondialyzable form and had a galactose-mannose ratio of 2:5. The complex containing 68% protein was separated into polysaccharide and glycoprotein components by ion-exchange chromatography. The galactomannan formed a single precipitate in immunodiffusion, sharing a common antigen with the glycoprotein which contained two precipitating antigens. The glycoprotein elicited delayed-type hypersensitivity in guinea pigs as measured by skin-test and macrophage migration inhibitory factor assay, with 5 μg resulting in 86% specific inhibition. The galactomannan was skin-test negative, but 10 μg resulted in 80% specific inhibition of migration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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