Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1974 Oct;10(4):816–822. doi: 10.1128/iai.10.4.816-822.1974

Growth of Typhoid and Paratyphoid Bacilli in Intravenously Infected Mice

Philip B Carter 1, Frank M Collins 1
PMCID: PMC423027  PMID: 4214784

Abstract

The in vivo growth of Salmonella paratyphi A, S. paratyphi B, S. paratyphi C, and S. typhi, as well as of an S. typhi-typhimurium hybrid, was studied in three different strains of mice. S. paratyphi A and B and S. typhi demonstrated very little growth potential in any of the intravenously infected mice, even after as many as 20 serial mouse passages. It was noted, however, that small numbers of viable S. paratyphi B and S. typhi persisted in the spleens of infected mice for up to 28 days. Salmonella paratyphi C and the S. typhi-typhimurium hybrid gave rise to progressive systemic infections beginning from very small intravenous inocula. The median lethal doses for the C57B1 strain of mouse were about five organisms. The relevance of these findings with regard to the development of an animal model for studying human typhoid fever vaccines is discussed.

Full text

PDF
816

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Collins F. M., Carter P. B. Comparative immunogenicity of heat-killed and living oral Salmonella vaccines. Infect Immun. 1972 Oct;6(4):451–458. doi: 10.1128/iai.6.4.451-458.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Collins F. M. Immunity to enteric infection in mice. Infect Immun. 1970 Mar;1(3):243–250. doi: 10.1128/iai.1.3.243-250.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Collins F. M. Immunogenicity of living and heat-killed Salmonella pullorum vaccines. Infect Immun. 1973 May;7(5):735–742. doi: 10.1128/iai.7.5.735-742.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Collins F. M., Mackaness G. B., Blanden R. V. Infection-immunity in experimental salmonellosis. J Exp Med. 1966 Oct 1;124(4):601–619. doi: 10.1084/jem.124.4.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collins F. M. Mechanisms in antimicrobial immunity. J Reticuloendothel Soc. 1971 Jul;10(1):58–99. [PubMed] [Google Scholar]
  6. Collins F. M. Salmonellosis in orally infected specific pathogen-free C57B1 mice. Infect Immun. 1972 Feb;5(2):191–198. doi: 10.1128/iai.5.2.191-198.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Diena B. B., Johnson E. M., Baron L. S., Wallace R., Greenberg L. Assay of typhoid vaccines with Salmonella typhosa-Salmonella typhimurium hybrids. Infect Immun. 1973 Jan;7(1):5–8. doi: 10.1128/iai.7.1.5-8.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. EDSALL G., GAINES S., LANDY M., TIGERTT W. D., SPRINZ H., TRAPANI R. J., MANDEL A. D., BENENSON A. S. Studies on infection and immunity in experimental typhoid fever. I. Typhoid fever in chimpanzees orally infected with Salmonella typhosa. J Exp Med. 1960 Jul 1;112:143–166. doi: 10.1084/jem.112.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Esposito V. M., Feeley J. C., Leeder W. D., Pittman M. Immunological response of three mouse strains to typhoid vaccine and Vi antigen. J Bacteriol. 1969 Jul;99(1):8–12. doi: 10.1128/jb.99.1.8-12.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Frost A. J., Smith H., Witt K., Keppie J. The chemical basis of the virulence of Brucella abortus. X. A surface virulence factor which facilitates intracellular growth of Brucella abortus in bovine phagocytes. Br J Exp Pathol. 1972 Dec;53(6):587–596. [PMC free article] [PubMed] [Google Scholar]
  11. GERICHTER C. B. The dissemination of Salmonella typhi, S. paratyphi A and S. paratyphi B through the organs of the white mouse by oral infection. J Hyg (Lond) 1960 Sep;58:307–319. doi: 10.1017/s0022172400038420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HOWARD J. G. Resistance to infection with Salmonella paratyphi C in mice parasitized with a relatively avirulent strain of Salmonella typhimurium. Nature. 1961 Jul 1;191:87–88. doi: 10.1038/191087a0. [DOI] [PubMed] [Google Scholar]
  13. Hornick R. B., Woodward T. E. Appraisal of typhoid vaccine in experimentally infected human subjects. Trans Am Clin Climatol Assoc. 1967;78:70–78. [PMC free article] [PubMed] [Google Scholar]
  14. LANDY M., GAINES S., SPRINZ H. Studies on intracerebral typhoid infection in mice. I. Characteristics of the infection. Br J Exp Pathol. 1957 Feb;38(1):15–24. [PMC free article] [PubMed] [Google Scholar]
  15. Mackaness G. B., Blanden R. V., Collins F. M. Host-parasite relations in mouse typhoid. J Exp Med. 1966 Oct 1;124(4):573–583. doi: 10.1084/jem.124.4.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mackaness G. B. The influence of immunologically committed lymphoid cells on macrophage activity in vivo. J Exp Med. 1969 May 1;129(5):973–992. doi: 10.1084/jem.129.5.973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pittman M., Bohner H. J. Laboratory assays of different types of field trial typhoid vaccines and relationship to efficacy in man. J Bacteriol. 1966 May;91(5):1713–1723. doi: 10.1128/jb.91.5.1713-1723.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. RAUSS K., KETYI I. Application of the quantitative bacteriaemia test according to Lipp in the determination of potency of typhoid antigens. Z Immun exp ther. 1962 Jul;123:173–183. [PubMed] [Google Scholar]
  19. Robson H. G., Vas S. I. Resistance of inbred mice to Salmonella typhimurium. J Infect Dis. 1972 Oct;126(4):378–386. doi: 10.1093/infdis/126.4.378. [DOI] [PubMed] [Google Scholar]
  20. SPAUN J. STUDIES ON THE INFLUENCE OF THE ROUTE OF IMMUNIZATION IN THE ACTIVE MOUSE PROTECTION TEST WITH INTRAPERITONEAL CHALLENGE FOR POTENCY ASSAY OF TYPHOID VACCINES. Bull World Health Organ. 1964;31:793–798. [PMC free article] [PubMed] [Google Scholar]
  21. SPAUN J. Typhoid vaccine; Vi antigen and Vi antibody. Acta Pathol Microbiol Scand Suppl. 1957;123:1–79. [PubMed] [Google Scholar]
  22. STANDFAST A. F. A report on the laboratory assays carried out at the Lister Institute of Preventive Medicine on the typhoid vaccines used in the field study in Yugoslavia. Bull World Health Organ. 1960;23:37–45. [PMC free article] [PubMed] [Google Scholar]
  23. Volkman A., Collins F. M. Polyarthritis associated with Salmonella infection in rats. Infect Immun. 1973 Nov;8(5):814–818. doi: 10.1128/iai.8.5.814-818.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WOODWARD T. E. TYPHOID FEVER: A DISCUSSION OF ITS PATHOGENESIS, PHYSIOLOGIC ABNORMALITIES AND MANAGEMENT. Trans Stud Coll Physicians Phila. 1963 Jul;31:26–39. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES