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. 1974 Oct;10(4):872–876. doi: 10.1128/iai.10.4.872-876.1974

Ultrastructure of Pili and Annular Structures on the Cell Wall Surface of Neisseria meningitidis

I W Devoe 1, J E Gilchrist 1
PMCID: PMC423034  PMID: 4139121

Abstract

In a survey of negatively stained preparations of the prototype strains of Neisseria meningitidis, pili were detected on three strains. However, these pili were detected on fewer than 5% of cells in populations of these three strains. Those individual cells with pili were seldom observed to contain more than two or three pili per cell. In contrast, nearly all cells of the nonprototype group B strain ATCC 13090 had numerous pili on their surfaces. When viewed in frozen-etched replicas, a few pili were observed lying on the cell surface of this latter strain. An annular structure was also found in frozen-etched replicas. This structure usually consisted of a series of concentric rings that were always found on the flat side of these bean-shaped cells. It is concluded that such structures represent a differentiated portion of the cell wall, which is involved in cross-wall formation during synthesis of the cell septum in dividing cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cesarini J. P., Vandekerkove M., Faucon R., Nicoli J. Ultrastructure de la paroi de Neisseria meningitidis. Ann Inst Pasteur (Paris) 1967 Dec;113(6):833–841. [PubMed] [Google Scholar]
  2. DeVoe I. W., Gilchrist J. E., Storm D. W. Ultrastructural studies on the fate of group B meningococci in human peripheral blood leukocytes. Can J Microbiol. 1973 Nov;19(11):1355–1359. doi: 10.1139/m73-218. [DOI] [PubMed] [Google Scholar]
  3. Devoe I. W., Gilchrist J. E. Release of endotoxin in the form of cell wall blebs during in vitro growth of Neisseria meningitidis. J Exp Med. 1973 Nov 1;138(5):1156–1167. doi: 10.1084/jem.138.5.1156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ducker T. B., Simmons R. L. The pathogenesis of meningitis. Systemic effect of meningococcal endotoxin within the cerebrospinal fluid. Arch Neurol. 1968 Feb;18(2):123–128. doi: 10.1001/archneur.1968.00470320025002. [DOI] [PubMed] [Google Scholar]
  5. Jephcott A. E., Reyn A., Birch-Andersen A. Neisseria gonorrhoeae 3. Demonstration of presumed appendages to cells from different colony types. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(3):437–439. doi: 10.1111/j.1699-0463.1971.tb00086.x. [DOI] [PubMed] [Google Scholar]
  6. KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. SCHERP H. W. Neisseria and neisserial infections. Annu Rev Microbiol. 1955;9:319–334. doi: 10.1146/annurev.mi.09.100155.001535. [DOI] [PubMed] [Google Scholar]
  8. Swanson J., Goldschneider I. The serum bactericidal system: ultrastructural changes in Neisseria meningitidis exposed to normal rat serum. J Exp Med. 1969 Jan 1;129(1):51–79. doi: 10.1084/jem.129.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Swanson J., Kraus S. J., Gotschlich E. C. Studies on gonococcus infection. I. Pili and zones of adhesion: their relation to gonococcal growth patterns. J Exp Med. 1971 Oct 1;134(4):886–906. doi: 10.1084/jem.134.4.886. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Swanson J. Studies on gonococcus infection. II. Freeze-fracture, freeze-etch studies on gonocci. J Exp Med. 1972 Nov 1;136(5):1258–1271. doi: 10.1084/jem.136.5.1258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Swanson J. Studies on gonococcus infection. IV. Pili: their role in attachment of gonococci to tissue culture cells. J Exp Med. 1973 Mar 1;137(3):571–589. doi: 10.1084/jem.137.3.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thompson J., DeVoe I. W. Physiological and morphological effects of phenethyl alcohol upon a gram-negative marine pseudomonad. Can J Microbiol. 1972 Jun;18(6):841–852. doi: 10.1139/m72-130. [DOI] [PubMed] [Google Scholar]
  13. Wistreich G. A., Baker R. F. The presence of fimbriae (pili) in three species of Neisseria. J Gen Microbiol. 1971 Feb;65(2):167–173. doi: 10.1099/00221287-65-2-167. [DOI] [PubMed] [Google Scholar]

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