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. 1974 Nov;10(5):1091–1097. doi: 10.1128/iai.10.5.1091-1097.1974

Immunogenicity of Ribosomal Preparations from Yeast Cells of Histoplasma capsulatum

Carl Feit 1, Ram P Tewari 1
PMCID: PMC423067  PMID: 16558095

Abstract

Protective immunity was elicited by immunization of mice with ribosomal preparations from yeast cells of Histoplasma capsulatum. Ribosomes from disrupted cells were isolated by differential centrifugation using sodium dodecyl sulfate. These preparations contained 55% protein and 45% ribonucleic acid and sedimented as a single peak with a sedimentation coefficient of 77S on sucrose density gradient analysis. Mice immunized subcutaneously with ribosomes, with or without adjuvant, were challenged intravenously with 8 × 106 yeast cells of H. capsulatum. Significant protection was induced by ribosomes and was greatly enhanced by adjuvants. Protection measured by 30-day survival compared favorably with the immunoprotection assessed by absence of lung lesions and negative spleen cultures. Treatment of ribosomes with ribonuclease before immunization reduced protection by 85%, whereas trypsin and Pronase reduced the protection by 50 to 55%. These findings indicate that both intact ribosomal ribonucleic acid and protein are necessary for maximal immunogenicity of Histoplasma ribosomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson K. L., Marcus S. Immunity to histoplasmosis induced in mice by components of Histoplasma capsulatum. Am Rev Respir Dis. 1970 Oct;102(4):614–622. doi: 10.1164/arrd.1970.102.4.614. [DOI] [PubMed] [Google Scholar]
  2. Bauman D. S., Chick E. W. Acute cavitary histoplasmosis in Rhesus monkeys: influence of immunological status. Infect Immun. 1973 Aug;8(2):245–248. doi: 10.1128/iai.8.2.245-248.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DISCHE Z. New color reactions for determination of sugars in polysaccharides. Methods Biochem Anal. 1955;2:313–358. doi: 10.1002/9780470110188.ch11. [DOI] [PubMed] [Google Scholar]
  4. Garvey M. C., Tewari R. P. A cell-free amino acid incorporating system from yeast-phase cells of Histoplasma capsulatum. Sabouraudia. 1972 Jul;10(2):113–121. doi: 10.1080/00362177285190241. [DOI] [PubMed] [Google Scholar]
  5. HILLEMAN M. R. A FORWARD LOOK AT VIRAL VACCINES: WITH SPECIAL REFERENCE TO A NEW IMMUNOLOGIC ADJUVANT. Am Rev Respir Dis. 1964 Nov;90:683–706. doi: 10.1164/arrd.1964.90.5.683. [DOI] [PubMed] [Google Scholar]
  6. Jensen R., Gregory B., Naylor J., Actor P. Isolation of protective somatic antigen from Vibrio cholerae (Ogawa) ribosomal preparations. Infect Immun. 1972 Aug;6(2):156–161. doi: 10.1128/iai.6.2.156-161.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Johnson W. Ribosomal vaccines. I. Immunogenicity of ribosomal fractions isolated from Salmonella typhimurium and Yersinia pestis. Infect Immun. 1972 Jun;5(6):947–952. doi: 10.1128/iai.5.6.947-952.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kellems R. E., Butow R. A. Cytoplasmic-type 80 S ribosomes associated with yeast mitochondria. I. Evidence for ribosome binding sites on yeast mitochondria. J Biol Chem. 1972 Dec 25;247(24):8043–8050. [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  11. Oka Y., Ito Y., Shinzato J., Osaki H. [Analysis of immunogenicity in the protozoan cell. 20. Protective antigenicity of the membrane structure and ribosomes isolated from the Trichomonas foetus microsomes]. Igaku To Seibutsugaku. 1967 Jul 10;75(1):17–20. [PubMed] [Google Scholar]
  12. Preston P. M., Dumonde D. C. Immunogenicity of a ribosomal antigen of Leishmania enriettii. Trans R Soc Trop Med Hyg. 1971;65(1):18–19. [PubMed] [Google Scholar]
  13. SALVIN S. B., RIBI E. Antigens from yeast phase of Histoplasma capsulatum. II. Immunologic properties of protoplasm vs. cell walls. Proc Soc Exp Biol Med. 1955 Oct;90(1):287–294. doi: 10.3181/00379727-90-22012. [DOI] [PubMed] [Google Scholar]
  14. SALVIN S. B., SMITH R. F. Antigens from the yeast phase of Histoplasma capsulatum. III. Isolation, properties, and activity of a protein-carbohydrate complex. J Infect Dis. 1959 Jul-Aug;105(1):45–53. doi: 10.1093/infdis/105.1.45. [DOI] [PubMed] [Google Scholar]
  15. SASLAW S., SCHAEFER J. Survival of Histoplasma capsulatum in experimental histoplasmosis in mice. Proc Soc Exp Biol Med. 1956 Mar;91(3):412–414. doi: 10.3181/00379727-91-22279. [DOI] [PubMed] [Google Scholar]
  16. Salvin S. B. Constituents of the cell wall of the yeast phase of Histoplasma capsulatum. Am Rev Respir Dis. 1965 Dec;92(6):119–125. doi: 10.1164/arrd.1965.92.6P2.119. [DOI] [PubMed] [Google Scholar]
  17. Smith R. A., Bigley N. J. Ribonucleic acid-protein fractions of virulent Salmonella typhimurium as protective immunogens. Infect Immun. 1972 Sep;6(3):377–383. doi: 10.1128/iai.6.3.377-383.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tewari R. P., Berkhout F. J. Comparative pathogenicity of albino and brown types of Histoplasma capsulatum for mice. J Infect Dis. 1972 May;125(5):504–508. doi: 10.1093/infdis/125.5.504. [DOI] [PubMed] [Google Scholar]
  19. Tewari R. P., Macpherson C. R. Cross-protection between Histoplasma capsulatum and Oidiodendron kalrai in mice. Mycopathol Mycol Appl. 1969 Jan 29;37(1):97–103. doi: 10.1007/BF02051338. [DOI] [PubMed] [Google Scholar]
  20. Thomas D. W., Weiss E. Response of mice to injection of ribosomal fraction from group B Neisseria meningitidis. Infect Immun. 1972 Sep;6(3):355–363. doi: 10.1128/iai.6.3.355-363.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Thompson H. C., Snyder I. S. Protection against pneumococcal infection by a ribosomal preparation. Infect Immun. 1971 Jan;3(1):16–23. doi: 10.1128/iai.3.1.16-23.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Venneman M. R., Bigley N. J., Berry L. J. Immunogenicity of Ribonucleic Acid Preparations Obtained from Salmonella typhimurium. Infect Immun. 1970 Jun;1(6):574–582. doi: 10.1128/iai.1.6.574-582.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Venneman M. R., Bigley N. J. Isolation and partial characterization of an immunogenic moiety obtained from Salmonella typhimurium. J Bacteriol. 1969 Oct;100(1):140–148. doi: 10.1128/jb.100.1.140-148.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Winston S. H., Berry L. J. Antibacterial immunity induced by ribosomal vaccines. J Reticuloendothel Soc. 1970 Jul;8(1):13–24. [PubMed] [Google Scholar]
  25. Winston S., Berry L. J. Immunity induced by ribosomal extracts from Staphylococcus aureus. J Reticuloendothel Soc. 1970 Jul;8(1):66–73. [PubMed] [Google Scholar]
  26. YOUMANS A. S., YOUMANS G. P. IMMUNOGENIC ACTIVITY OF A RIBOSOMAL FRACTION OBTAINED FROM MYCOBACTERIUM TUBERCULOSIS. J Bacteriol. 1965 May;89:1291–1298. doi: 10.1128/jb.89.5.1291-1298.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Youmans A. S., Youmans G. P. Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Mycobacterium tuberculosis. J Bacteriol. 1966 Jun;91(6):2146–2154. doi: 10.1128/jb.91.6.2146-2154.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Youmans A. S., Youmans G. P. Factors affecting immunogenic activity of mycobacterial ribosomal and ribonucleic acid preparations. J Bacteriol. 1969 Jul;99(1):42–50. doi: 10.1128/jb.99.1.42-50.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Youmans A. S., Youmans G. P. Failure of synthetic polynucleotides to affect the immunogenicity of mycobacterial ribonucleic Acid and ribosomal protein preparations. Infect Immun. 1971 Jan;3(1):149–153. doi: 10.1128/iai.3.1.149-153.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Youmans A. S., Youmans G. P. Immunogenic mycobacterial ribosomal and ribonucleic Acid preparations: chemical and physical characteristics. Infect Immun. 1970 Nov;2(5):659–668. doi: 10.1128/iai.2.5.659-668.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Youmans A. S., Youmans G. P. Preparation and effect of different adjuvants on the immunogenic activity of mycobacterial ribosomal fraction. J Bacteriol. 1967 Oct;94(4):836–843. doi: 10.1128/jb.94.4.836-843.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

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