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. 1974 Nov;10(5):1170–1179. doi: 10.1128/iai.10.5.1170-1179.1974

Ultrastructure of Mutants of Streptococcus mutans with Reference to Agglutination, Adhesion, and Extracellular Polysaccharide

J Nalbandian 1, M L Freedman 1, J M Tanzer 1, S M Lovelace 1
PMCID: PMC423078  PMID: 16558106

Abstract

An electron microscopic study of S. mutans 6715-13 wild type and representatives of three distinct classes of glucan synthesis-defective mutants (which fail to form adherent microbial plaques but agglutinate normally in the presence of exogenous glucans) disclosed the presence of two sucrose-dependent, glucanase-sensitive, extracellular components. In the wild type, these extracellular glucans had predominantly fibrillar (with some globular) morphologies. However, in the mutant strains, there was a consistent reduction in or loss of the fibrillar components and dramatic increases in globular forms. A cell surface-associated fuzzy coat was consistently seen, and it was neither sucrose-dependent nor glucanase-sensitive. The data indicated that in vitro and in vivo adhesion and virulence at smooth tooth surfaces (all these properties dramatically reduced in the mutants) were causally and functionally related to the extracellular, fibrillar, glucan component, whereas in vitro glucan-mediated agglutination may be related to the cell-associated surface fuzzy coat.

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Selected References

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  1. Bratthall D. Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odontol Revy. 1970;21(2):143–152. [PubMed] [Google Scholar]
  2. Chludzinski A. M., Germaine G. R., Schachtele C. F. Purification and properties of dextransucrase from Streptococcus mutans. J Bacteriol. 1974 Apr;118(1):1–7. doi: 10.1128/jb.118.1.1-7.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coykendall A. L. Genetic heterogeneity in Streptococcus mutans. J Bacteriol. 1971 Apr;106(1):192–196. doi: 10.1128/jb.106.1.192-196.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edwardsson S. Characteristics of caries-inducing human streptococci resembling Streptococcus mutans. Arch Oral Biol. 1968 Jun;13(6):637–646. doi: 10.1016/0003-9969(68)90142-8. [DOI] [PubMed] [Google Scholar]
  5. Edwardsson S. The caries-inducing property of variants of Streptococcus mutans. Odontol Revy. 1970;21(2):153–157. [PubMed] [Google Scholar]
  6. Ellen R. P., Gibbons R. J. M protein-associated adherence of Streptococcus pyogenes to epithelial surfaces: prerequisite for virulence. Infect Immun. 1972 May;5(5):826–830. doi: 10.1128/iai.5.5.826-830.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FITZGERALD R. J., KEYES P. H. Demonstration of the etiologic role of streptococci in experimental caries in the hamster. J Am Dent Assoc. 1960 Jul;61:9–19. doi: 10.14219/jada.archive.1960.0138. [DOI] [PubMed] [Google Scholar]
  8. Freedman M. L., Tanzer J. M. Dissociation of plaque formation from glucan-induced agglutination in mutants of Streptococcus mutans. Infect Immun. 1974 Jul;10(1):189–196. doi: 10.1128/iai.10.1.189-196.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gibbons R. J., Berman K. S., Knoettner P., Kapsimalis B. Dental caries and alveolar bone loss in gnotobiotic rats infected with capsule forming streptococci of human origin. Arch Oral Biol. 1966 Jun;11(6):549–560. doi: 10.1016/0003-9969(66)90220-2. [DOI] [PubMed] [Google Scholar]
  10. Gibbons R. J., Fitzgerald R. J. Dextran-induced agglutination of Streptococcus mutans, and its potential role in the formation of microbial dental plaques. J Bacteriol. 1969 May;98(2):341–346. doi: 10.1128/jb.98.2.341-346.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gibbons R. J., Nygaard M. Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci. Arch Oral Biol. 1968 Oct;13(10):1249–1262. doi: 10.1016/0003-9969(68)90081-2. [DOI] [PubMed] [Google Scholar]
  12. Gibbons R. J., Van Houte J., Liljemark W. F. Parameters that effect the adherence of Streptococcus salivarius to oral epithelial surfaces. J Dent Res. 1972 Mar-Apr;51(2):424–435. doi: 10.1177/00220345720510023101. [DOI] [PubMed] [Google Scholar]
  13. Guggenheim B. Enzymatic hydrolysis and structure of water-insoluble glucan produced by glucosyltransferases from a strain of streptococcus mutans. Helv Odontol Acta. 1970 Nov;14(Suppl):89+–89+. [PubMed] [Google Scholar]
  14. Guggenheim B. Extracellular polysaccharides and microbial plaque. Int Dent J. 1970 Dec;20(4):657–678. [PubMed] [Google Scholar]
  15. Guggenheim B., Newbrun E. Extracellular glucosyltransferase activity of an HS strain of Streptococcus mutans. Helv Odontol Acta. 1969 Oct;13(2):84–97. [PubMed] [Google Scholar]
  16. Guggenheim B., Schroeder H. E. Biochemical and morphological aspects of extracellular polysaccharides produced by cariogenic streptococci. Helv Odontol Acta. 1967 Oct;11(2):131–152. [PubMed] [Google Scholar]
  17. JORDAN H. V., FITZGERALD R. J., BOWLER A. E. Inhibition of experimental caries by sodium metabisulfite and its effect on the growth and metabolism of selected bacteria. J Dent Res. 1960 Jan-Feb;39:116–123. doi: 10.1177/00220345600390010501. [DOI] [PubMed] [Google Scholar]
  18. Johnson M. C., Bozzola J. J., Shechmeister I. L. Morphological study of Streptococcus mutans and two extracellular polysaccharide mutants. J Bacteriol. 1974 Apr;118(1):304–311. doi: 10.1128/jb.118.1.304-311.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Littleton N. W., Kakehashi S., Fitzgerald R. J. Recovery of specific "caries-inducing" streptococci from carious lesions in the teeth of children. Arch Oral Biol. 1970 May;15(5):461–463. doi: 10.1016/0003-9969(70)90073-7. [DOI] [PubMed] [Google Scholar]
  21. McCabe M. M., Smith E. E. Origin of the cell-associated dextransucrase of Streptococcus mutans. Infect Immun. 1973 Jun;7(6):829–838. doi: 10.1128/iai.7.6.829-838.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mukasa H., Slade H. D. Mechanism of adherence of Streptococcus mutans to smooth surfaces. I. Roles of insoluble dextran-levan synthetase enzymes and cell wall polysaccharide antigen in plaque formation. Infect Immun. 1973 Oct;8(4):555–562. doi: 10.1128/iai.8.4.555-562.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Newbrun E., Lacy R., Christie T. M. The morphology and size of the extracellular polysaccharides from oral streptococci. Arch Oral Biol. 1971 Aug;16(8):863–872. doi: 10.1016/0003-9969(71)90176-2. [DOI] [PubMed] [Google Scholar]
  24. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Robrish S. A., Reid W., Krichevsky M. I. Distribution of enzymes forming polysaccharide from sucrose and the composition of extracellular polysaccharide synthesized by Streptococcus mutans. Appl Microbiol. 1972 Aug;24(2):184–190. doi: 10.1128/am.24.2.184-190.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Swanson J., Hsu K. C., Gotschlich E. C. Electron microscopic studies on streptococci. I. M antigen. J Exp Med. 1969 Nov 1;130(5):1063–1091. doi: 10.1084/jem.130.5.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tanzer J. M., Freedman M. L., Fitzgerald R. J., Larson R. H. Diminished virulence of glucan synthesis-defective mutants of Streptococcus mutans. Infect Immun. 1974 Jul;10(1):197–203. doi: 10.1128/iai.10.1.197-203.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. ZINNER D. D., JABLON J. M., ARAN A. P., SASLAW M. S. EXPERIMENTAL CARIES INDUCED IN ANIMALS BY STREPTOCOCCI OF HUMAN ORIGIN. Proc Soc Exp Biol Med. 1965 Mar;118:766–770. doi: 10.3181/00379727-118-29964. [DOI] [PubMed] [Google Scholar]
  29. de Stoppelaar J. D., König K. G., Plasschaert A. J., van der Hoeven J. S. Decreased cariogenicity of a mutant of Streptococcus mutans. Arch Oral Biol. 1971 Aug;16(8):971–975. doi: 10.1016/0003-9969(71)90186-5. [DOI] [PubMed] [Google Scholar]

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