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. 1974 Dec;10(6):1391–1396. doi: 10.1128/iai.10.6.1391-1396.1974

Augmentation of Glucose Transport in Macrophages After Particle Ingestion

Peter F Bonventre 1, Antony J Mukkada 1
PMCID: PMC423116  PMID: 4435960

Abstract

Guinea pig and mouse peritoneal macrophages in culture transport glucose by a specific, saturable system with characteristics compatible with facilitated diffusion. Phagocytosis of killed staphylococci or polystyrene latex spheres results in a significant increase in uptake of 2-deoxy-d-glucose. Reciprocal plot analysis showed that the Km values were lowered as a consequence of phagocytosis by a factor of between 2 and 3 in both cell types; Vmax values were not significantly changed. The nature of the intracellular sugar pool was analyzed and found to consist of free and phosphorylated 2-deoxy-d-glucose at a relatively constant ratio of 1:2 after periods of uptake between 1 and 20 min. Phagocytosis resulted in increased levels of both free and phosphorylated sugars in the cytoplasm. Since the Km values were lowered, augmented glucose uptake could not be accounted for by altered hexokinase activity. It was concluded that phagocytosis induces changes in the glucose transport system per se. The data are compatible with the metabolic changes known to be associated with particle ingestion by phagocytic cells. The mechanism by which glucose transport is augmented after loss of significant amounts of cell surface during the phagocytic process is not yet known.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DeChatelet L. R., McCall C. E., McPhail L. C. Inhibition of amino acid incorporation into protein of human neutrophils by phagocytosis. Infect Immun. 1973 Nov;8(5):791–795. doi: 10.1128/iai.8.5.791-795.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Elsbach P., Pettis P., Beckerdite S., Franson R. Effects of phagocytosis by rabbit granulocytes on macromolecular synthesis and degradation in different species of bacteria. J Bacteriol. 1973 Aug;115(2):490–497. doi: 10.1128/jb.115.2.490-497.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Griffin F. M., Jr, Silverstein S. C. Segmental response of the macrophage plasma membrane to a phagocytic stimulus. J Exp Med. 1974 Feb 1;139(2):323–336. doi: 10.1084/jem.139.2.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hawkins R. A., Berlin R. D. Purine transport in polymorphonuclear leukocytes. Biochim Biophys Acta. 1969 Mar 11;173(2):324–337. doi: 10.1016/0005-2736(69)90115-1. [DOI] [PubMed] [Google Scholar]
  5. Isselbacher K. J. Increased uptake of amino acids and 2-deoxy-D-glucose by virus-transformed cells in culture. Proc Natl Acad Sci U S A. 1972 Mar;69(3):585–589. doi: 10.1073/pnas.69.3.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Klebanoff S. J. Iodination of bacteria: a bactericidal mechanism. J Exp Med. 1967 Dec 1;126(6):1063–1078. doi: 10.1084/jem.126.6.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McRipley R. J., Sbarra A. J. Role of the phagocyte in host-parasite interactions. XI. Relationship between stimulated oxidative metabolism and hydrogen peroxide formation, and intracellular killing. J Bacteriol. 1967 Nov;94(5):1417–1424. doi: 10.1128/jb.94.5.1417-1424.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. OYAMA V. I., EAGLE H. Measurement of cell growth in tissue culture with a phenol reagent (folin-ciocalteau). Proc Soc Exp Biol Med. 1956 Feb;91(2):305–307. doi: 10.3181/00379727-91-22245. [DOI] [PubMed] [Google Scholar]
  9. Reed P. W. Glutathione and the hexose monophosphate shunt in phagocytizing and hydrogen peroxide-treated rat leukocytes. J Biol Chem. 1969 May 10;244(9):2459–2464. [PubMed] [Google Scholar]
  10. Romano A. H., Colby C. SV40 virus transformation of mouse 3T3 cells does not specifically enhance sugar transport. Science. 1973 Mar 23;179(4079):1238–1240. doi: 10.1126/science.179.4079.1238. [DOI] [PubMed] [Google Scholar]
  11. Rothstein A. Membrane phenomena. Annu Rev Physiol. 1968;30:15–72. doi: 10.1146/annurev.ph.30.030168.000311. [DOI] [PubMed] [Google Scholar]
  12. SBARRA A. J., KARNOVSKY M. L. The biochemical basis of phagocytosis. I. Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes. J Biol Chem. 1959 Jun;234(6):1355–1362. [PubMed] [Google Scholar]
  13. Tsan M. F., Berlin R. D. Effect of phagocytosis on membrane transport of nonelectrolytes. J Exp Med. 1971 Oct 1;134(4):1016–1035. doi: 10.1084/jem.134.4.1016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ukena T. E., Berlin R. D. Effect of colchicine and vinblastine on the topographical separation of membrane functions. J Exp Med. 1972 Jul 1;136(1):1–7. doi: 10.1084/jem.136.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Winkler H. H. A hexose-phosphate transport system in Escherichia coli. Biochim Biophys Acta. 1966 Mar 28;117(1):231–240. doi: 10.1016/0304-4165(66)90170-x. [DOI] [PubMed] [Google Scholar]

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