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. 1985 Jan;75(1):112–118. doi: 10.1172/JCI111662

Regional distribution and alterations of lectin binding to colorectal mucin in mucosal biopsies from controls and subjects with inflammatory bowel diseases.

L R Jacobs, P W Huber
PMCID: PMC423415  PMID: 3965499

Abstract

Glycoconjugate composition of colorectal goblet cell mucin was characterized according to the anatomical distribution of lectin-binding sites in mucosal biopsies from 35 control subjects and 55 patients with inflammatory bowel disease. 24 of the controls had mucosal inflammation on biopsy, without clinical evidence of inflammatory bowel disease. These inflamed controls showed a similar rate of presence of lectin-binding sites as the normal noninflamed group. In the controls, the frequency of binding of Ricinus communis agglutinin I to galactosyl residues was consistently higher than that found with either Ulex europaeus agglutinin I to fucosyl or Dolichus biflorus agglutinin to N-acetyl galactosyl groups. A significant proximal to distal gradient for Ulex europaeus agglutinin I binding sites was identified in the controls group. These binding sites were present four times more often in the proximal colon than in the distal colon (P less than 0.025). In the ulcerative and Crohn's colitis groups, this gradient effect was lost, predominantly as a result of decreased availability of fucosyl residues in the proximal colon. In the descending colon of Crohn's colitis tissues, there was a complete absence of Dolichus biflorus agglutinin binding sites compared with the 62.5% incidence in the control group (P less than 0.05). These results demonstrate that the expression of lectin-binding sites in human large intestinal goblet mucin is specifically altered in inflammatory bowel disease, indicating that there are changes in glycosylation of colorectal mucin consistent with alterations in goblet cell differentiation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boland C. R., Montgomery C. K., Kim Y. S. Alterations in human colonic mucin occurring with cellular differentiation and malignant transformation. Proc Natl Acad Sci U S A. 1982 Mar;79(6):2051–2055. doi: 10.1073/pnas.79.6.2051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clamp J. R. Chemical aspects of mucus. General considerations. Br Med Bull. 1978 Jan;34(1):25–27. doi: 10.1093/oxfordjournals.bmb.a071455. [DOI] [PubMed] [Google Scholar]
  3. Clamp J. R., Fraser G., Read A. E. Study of the carbohydrate content of mucus glycoproteins from normal and diseased colons. Clin Sci (Lond) 1981 Aug;61(2):229–234. doi: 10.1042/cs0610229. [DOI] [PubMed] [Google Scholar]
  4. Dawson P. A., Patel J., Filipe M. I. Variations in sialomucins in the mucosa of the large intestine in malignancy: a quantimet and statistical analysis. Histochem J. 1978 Sep;10(5):559–572. doi: 10.1007/BF01003137. [DOI] [PubMed] [Google Scholar]
  5. Eastwood G. L., Trier J. S. Epithelial cell renewal in cultured rectal biopsies in ulcerative colitis. Gastroenterology. 1973 Mar;64(3):383–390. [PubMed] [Google Scholar]
  6. Ehsanullah M., Filipe M. I., Gazzard B. Mucin secretion in inflammatory bowel disease: correlation with disease activity and dysplasia. Gut. 1982 Jun;23(6):485–489. doi: 10.1136/gut.23.6.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Etzler M. E. Lectins as probes in studies of intestinal glycoproteins and glycolipids. Am J Clin Nutr. 1979 Jan;32(1):133–138. doi: 10.1093/ajcn/32.1.133. [DOI] [PubMed] [Google Scholar]
  8. Filipe M. I., Dawson I. The diagnostic value of mucosubstances in rectal biopsies from patients with ulcerative colitis and Crohn's disease. Gut. 1970 Mar;11(3):229–234. doi: 10.1136/gut.11.3.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Filipe M. I. Mucin histochemistry in the detection of early malignancy in the colonic epithelium. Adv Exp Med Biol. 1977;89:413–422. doi: 10.1007/978-1-4613-4172-7_28. [DOI] [PubMed] [Google Scholar]
  10. Filipe M. I. Mucins in the human gastrointestinal epithelium: a review. Invest Cell Pathol. 1979 Jul-Sep;2(3):195–216. [PubMed] [Google Scholar]
  11. Filipe M. I., Mughal S., Bussey H. J. Patterns of mucus secretion in the colonic epithelium in familial polyposis. Invest Cell Pathol. 1980 Oct-Dec;3(4):329–343. [PubMed] [Google Scholar]
  12. Filipe M. I. Value of histochemical reactions for mucosubstances in the diagnosis of certain pathological conditions of the colon and rectum. Gut. 1969 Jul;10(7):577–586. doi: 10.1136/gut.10.7.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gad A. A histochemical study of human alimentary tract mucosubstances in health and disease. II. Inflammatory conditions. Br J Cancer. 1969 Mar;23(1):64–68. doi: 10.1038/bjc.1969.10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Goodman M. J., Kent P. W., Truelove S. C. Glucosamine synthetase activity of the colonic mucosa in ulcerative colitis and Crohn's disease. Gut. 1977 Mar;18(3):219–228. doi: 10.1136/gut.18.3.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Greco V., Lauro G., Fabbrini A., Torsoli A. Histochemistry of the colonic epithelial mucins in normal subjects and in patients with ulcerative colitis. A qualitative and histophotometric investigation. Gut. 1967 Oct;8(5):491–496. doi: 10.1136/gut.8.5.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jacobs L. R., De Fontes D., Cox K. L. Cytochemical localization of small intestinal glycoconjugates by lectin histochemistry in controls and subjects with cystic fibrosis. Dig Dis Sci. 1983 May;28(5):422–428. doi: 10.1007/BF02430531. [DOI] [PubMed] [Google Scholar]
  17. Kim Y. S., Isaacs R. Glycoprotein metabolism in inflammatory and neoplastic diseases of the human colon. Cancer Res. 1975 Aug;35(8):2092–2097. [PubMed] [Google Scholar]
  18. Phelps C. F. Biosynthesis of mucus glycoprotein. Br Med Bull. 1978 Jan;34(1):43–48. doi: 10.1093/oxfordjournals.bmb.a071456. [DOI] [PubMed] [Google Scholar]
  19. Podolsky D. K., Isselbacher K. J. Composition of human colonic mucin. Selective alteration in inflammatory bowel disease. J Clin Invest. 1983 Jul;72(1):142–153. doi: 10.1172/JCI110952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rogers C. M., Cooke K. B., Filipe M. I. Sialic acids of human large bowel mucosa: o-acylated variants in normal and malignant states. Gut. 1978 Jul;19(7):587–592. doi: 10.1136/gut.19.7.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Serafini E. P., Kirk A. P., Chambers T. J. Rate and pattern of epithelial cell proliferation in ulcerative colitis. Gut. 1981 Aug;22(8):648–652. doi: 10.1136/gut.22.8.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Teague R. H., Fraser D., Clamp J. R. Changes in monosaccharide content of mucous glycoproteins in ulcerative colitis. Br Med J. 1973 Jun 16;2(5867):645–646. doi: 10.1136/bmj.2.5867.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Variyam E. P., Hoskins L. C. Mucin degradation in human colon ecosystems. Degradation of hog gastric mucin by fecal extracts and fecal cultures. Gastroenterology. 1981 Oct;81(4):751–758. [PubMed] [Google Scholar]
  24. Yonezawa S., Nakamura T., Tanaka S., Maruta K., Nishi M., Sato E. Binding of Ulex europaeus agglutinin-I in polyposis coli: comparative study with solitary adenoma in the sigmoid colon and rectum. J Natl Cancer Inst. 1983 Jul;71(1):19–24. [PubMed] [Google Scholar]
  25. Yonezawa S., Nakamura T., Tanaka S., Sato E. Glycoconjugate with Ulex europaeus agglutinin-I-binding sites in normal mucosa, adenoma, and carcinoma of the human large bowel. J Natl Cancer Inst. 1982 Oct;69(4):777–785. [PubMed] [Google Scholar]

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