Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1985 Feb;75(2):722–725. doi: 10.1172/JCI111752

A monokine stimulates production of human erythroid burst-promoting activity by endothelial cells in vitro.

K S Zuckerman, G C Bagby Jr, E McCall, B Sparks, J Wells, V Patel, D Goodrum
PMCID: PMC423565  PMID: 3973026

Abstract

Conditioned media were prepared from human peripheral blood monocytes and human umbilical vein endothelial cells. These media were assayed for erythroid burst-promoting activity (BPA) using human peripheral blood monocyte-depleted mononuclear cells as targets and assessing the stimulatory effect of the conditioned media on growth of early erythroid progenitor cells. Both monocytes and endothelial cells produced modest amounts of detectable BPA. Addition of varying concentrations of media conditioned by monocytes to plateau concentrations (5-10%) of media conditioned by endothelial cells had no additive effect. Endothelial cells incubated in the presence of 50% monocyte-conditioned medium produced 2.5- to 6.6-fold more BPA than did endothelial cells incubated only in control tissue culture medium. In contrast, endothelial cell conditioned medium did not stimulate increased BPA production by monocytes. Neither neutrophil- nor marrow fibroblastoid cell-conditioned medium stimulated BPA production by endothelial cells. Therefore, both monocytes and endothelial cells produce BPA. Moreover, monocytes produce a monokine that, in turn, stimulates the production of BPA by endothelial cells. Inasmuch as a monokine also has been shown to stimulate production of granulocyte-macrophage colony-stimulating activity, we propose that monocytes play a critical role in regulating the production of humoral regulators of the very early stages of hemopoietic cell differentiation.

Full text

PDF
725

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ascensao J. L., Vercellotti G. M., Jacob H. S., Zanjani E. D. Role of endothelial cells in human hematopoiesis: modulation of mixed colony growth in vitro. Blood. 1984 Mar;63(3):553–558. [PubMed] [Google Scholar]
  2. Bagby G. C., Jr, McCall E., Bergstrom K. A., Burger D. A monokine regulates colony-stimulating activity production by vascular endothelial cells. Blood. 1983 Sep;62(3):663–668. [PubMed] [Google Scholar]
  3. Bagby G. C., Jr, McCall E., Layman D. L. Regulation of colony-stimulating activity production. Interactions of fibroblasts, mononuclear phagocytes, and lactoferrin. J Clin Invest. 1983 Feb;71(2):340–344. doi: 10.1172/JCI110774. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bagby G. C., Jr, Rigas V. D., Bennett R. M., Vandenbark A. A., Garewal H. S. Interaction of lactoferrin, monocytes, and T lymphocyte subsets in the regulation of steady-state granulopoiesis in vitro. J Clin Invest. 1981 Jul;68(1):56–63. doi: 10.1172/JCI110254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  6. Gordon L. I., Miller W. J., Branda R. F., Zanjani E. D., Jacob H. S. Regulation of erythroid colony formation by bone marrow macrophages. Blood. 1980 Jun;55(6):1047–1050. [PubMed] [Google Scholar]
  7. Gordon M. Y., Kearney L., Hibbin J. A. Effects of human marrow stromal cells on proliferation by human granulocytic (GM-CFC), erythroid (BFU-E) and mixed (Mix-CFC) colony-forming cells. Br J Haematol. 1983 Feb;53(2):317–325. doi: 10.1111/j.1365-2141.1983.tb02026.x. [DOI] [PubMed] [Google Scholar]
  8. Mangan K. F., Desforges J. F. The role of T lymphocytes and monocytes in the regulation of human erythropoietic peripheral blood burst forming units. Exp Hematol. 1980 Jul;8(6):717–727. [PubMed] [Google Scholar]
  9. Nathan D. G., Chess L., Hillman D. G., Clarke B., Breard J., Merler E., Housman D. E. Human erythroid burst-forming unit: T-cell requirement for proliferation in vitro. J Exp Med. 1978 Feb 1;147(2):324–339. doi: 10.1084/jem.147.2.324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Quesenberry P. J., Gimbrone M. A., Jr Vascular endothelium as a regulator of granulopoiesis: production of colony-stimulating activity by cultured human endothelial cells. Blood. 1980 Dec;56(6):1060–1067. [PubMed] [Google Scholar]
  11. Quesenberry P., Levitt L. Hematopoietic stem cells (second of three parts). N Engl J Med. 1979 Oct 11;301(15):819–823. doi: 10.1056/NEJM197910113011505. [DOI] [PubMed] [Google Scholar]
  12. Reid C. D., Baptista L. C., Chanarin I. Erythroid colony growth in vitro from human peripheral blood null cells: evidence for regulation by T-lymphocytes and monocytes. Br J Haematol. 1981 May;48(1):155–164. doi: 10.1111/j.1365-2141.1981.00155.x. [DOI] [PubMed] [Google Scholar]
  13. Torok-Storb B., Martin P. J., Hansen J. A. Regulation of in vitro erythropoiesis by normal T cells: evidence for two T-cell subsets with opposing function. Blood. 1981 Jul;58(1):171–174. [PubMed] [Google Scholar]
  14. Tsang R. W., Aye M. T. Evidence for proliferation of erythroid progenitor cells in the absence of added erythropoietin. Exp Hematol. 1979 Aug;7(7):383–388. [PubMed] [Google Scholar]
  15. Zuckerman K. S., Haak M. Mitogen-induced stimulation and suppression of erythroid burst promoting activity production by human mononuclear cells. Br J Haematol. 1983 Sep;55(1):145–153. doi: 10.1111/j.1365-2141.1983.tb01232.x. [DOI] [PubMed] [Google Scholar]
  16. Zuckerman K. S. Human erythroid burst-forming units. Growth in vitro is dependent on monocytes, but not T lymphocytes. J Clin Invest. 1981 Mar;67(3):702–709. doi: 10.1172/JCI110086. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Zuckerman K. S., Patel V. R., Goodrum D. D. Production of human erythroid burst promoting activity by monocytes stimulated with bacterial lipopolysaccharide. Exp Hematol. 1983 Jul;11(6):475–480. [PubMed] [Google Scholar]
  18. Zuckerman K. S. Stimulation of human BFU(E) by products of human monocytes and lymphocytes. Exp Hematol. 1980 Aug;8(7):924–932. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES