Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1986 Nov;78(5):1397–1404. doi: 10.1172/JCI112727

Epitopes of apolipoprotein B-100 and B-48 in both liver and intestine. Expression and evidence for local synthesis in recessive abetalipoproteinemia.

R P Dullaart, B Speelberg, H J Schuurman, R W Milne, L M Havekes, Y L Marcel, H J Geuze, M M Hulshof, D W Erkelens
PMCID: PMC423844  PMID: 2429992

Abstract

The presence of apolipoprotein (apo) B in liver and intestine from a patient with abetalipoproteinemia was evaluated by immunohistochemistry with a polyclonal and six monoclonal antibodies to different apo B-48 and B-100 epitopes. In normal liver, apo B was present inside and outside hepatocytes. The patients liver exhibited staining in the cytoplasm with the polyclonal and three monoclonal antibodies. By immunoelectron-microscopy, apo B was found to be present in the smooth endoplasmatic reticulum and the Golgi complex. Normal intestinal epithelium was labeled with polyclonal and all monoclonal antibodies, including those specific for apo B-100. The patients epithelium stained with polyclonal and six monoclonals, and apo B was present in the Golgi complex. Thus, normal intestinal mucosa expressed apo B-48 and B-100 epitopes, which indicates apo B-100 synthesis in the gut. The synthesis of the apo B molecule in the patient seems to be retained in both liver and gut, which suggests a posttranslational defect.

Full text

PDF
1402

Images in this article

1399Image
on p.1399
1399Image
on p.1399
1399Image
on p.1399
1400Image
on p.1400
1400Image
on p.1400
1401Image
on p.1401
1401Image
on p.1401
1402Image
on p.1402

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander C. A., Hamilton R. L., Havel R. J. Subcellular localization of B apoprotein of plasma lipoproteins in rat liver. J Cell Biol. 1976 May;69(2):241–263. doi: 10.1083/jcb.69.2.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BASSEN F. A., KORNZWEIG A. L. Malformation of the erythrocytes in a case of atypical retinitis pigmentosa. Blood. 1950 Apr;5(4):381–387. [PubMed] [Google Scholar]
  3. Christensen N. J., Rubin C. E., Cheung M. C., Albers J. J. Ultrastructural immunolocalization of apolipoprotein B within human jejunal absorptive cells. J Lipid Res. 1983 Sep;24(9):1229–1242. [PubMed] [Google Scholar]
  4. Cottrill C., Glueck C. J., Leuba V., Millett F., Puppione D., Brown W. V. Familial homozygous hypobetalipoproteinemia. Metabolism. 1974 Aug;23(8):779–791. doi: 10.1016/0026-0495(74)90010-9. [DOI] [PubMed] [Google Scholar]
  5. Geuze H. J., Slot J. W., van der Ley P. A., Scheffer R. C. Use of colloidal gold particles in double-labeling immunoelectron microscopy of ultrathin frozen tissue sections. J Cell Biol. 1981 Jun;89(3):653–665. doi: 10.1083/jcb.89.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Glickman R. M., Green P. H., Lees R. S., Lux S. E., Kilgore A. Immunofluorescence studies of apolipoprotein B in intestinal mucosa. Absence in abetalipoproteinemia. Gastroenterology. 1979 Feb;76(2):288–292. [PubMed] [Google Scholar]
  7. Glickman R. M., Khorana J., Kilgore A. Localization of apolipoprotein B in intestinal epithelial cells. Science. 1976 Sep 24;193(4259):1254–1255. doi: 10.1126/science.183265. [DOI] [PubMed] [Google Scholar]
  8. Hegele R. A., Angel A. Arrest of neuropathy and myopathy in abetalipoproteinemia with high-dose vitamin E therapy. Can Med Assoc J. 1985 Jan 1;132(1):41–44. [PMC free article] [PubMed] [Google Scholar]
  9. Herbert P. N., Hyams J. S., Bernier D. N., Berman M. M., Saritelli A. L., Lynch K. M., Nichols A. V., Forte T. M. Apolipoprotein B-100 deficiency. Intestinal steatosis despite apolipoprotein B-48 synthesis. J Clin Invest. 1985 Aug;76(2):403–412. doi: 10.1172/JCI111986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kane J. P. Apolipoprotein B: structural and metabolic heterogeneity. Annu Rev Physiol. 1983;45:637–650. doi: 10.1146/annurev.ph.45.030183.003225. [DOI] [PubMed] [Google Scholar]
  11. Kane J. P., Hardman D. A., Paulus H. E. Heterogeneity of apolipoprotein B: isolation of a new species from human chylomicrons. Proc Natl Acad Sci U S A. 1980 May;77(5):2465–2469. doi: 10.1073/pnas.77.5.2465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kayden H. J. Abetalipoproteinemia. Annu Rev Med. 1972;23:285–296. doi: 10.1146/annurev.me.23.020172.001441. [DOI] [PubMed] [Google Scholar]
  13. Krishnaiah K. V., Walker L. F., Borensztajn J., Schonfeld G., Getz G. S. Apolipoprotein B variant derived from rat intestine. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3806–3810. doi: 10.1073/pnas.77.7.3806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mahley R. W., Innerarity T. L., Rall S. C., Jr, Weisgraber K. H. Plasma lipoproteins: apolipoprotein structure and function. J Lipid Res. 1984 Dec 1;25(12):1277–1294. [PubMed] [Google Scholar]
  15. Malloy M. J., Kane J. P., Hardman D. A., Hamilton R. L., Dalal K. B. Normotriglyceridemic abetalipoproteinemia. absence of the B-100 apolipoprotein. J Clin Invest. 1981 May;67(5):1441–1450. doi: 10.1172/JCI110173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Malloy M. J., Kane J. P. Hypolipidemia. Med Clin North Am. 1982 Mar;66(2):469–484. doi: 10.1016/s0025-7125(16)31431-6. [DOI] [PubMed] [Google Scholar]
  17. Marcel Y. L., Hogue M., Theolis R., Jr, Milne R. W. Mapping of antigenic determinants of human apolipoprotein B using monoclonal antibodies against low density lipoproteins. J Biol Chem. 1982 Nov 25;257(22):13165–13168. [PubMed] [Google Scholar]
  18. Milne R. W., Theolis R., Jr, Verdery R. B., Marcel Y. L. Characterization of monoclonal antibodies against human low density lipoprotein. Arteriosclerosis. 1983 Jan-Feb;3(1):23–30. doi: 10.1161/01.atv.3.1.23. [DOI] [PubMed] [Google Scholar]
  19. Muller D. P., Lloyd J. K., Bird A. C. Long-term management of abetalipoproteinaemia. Possible role for vitamin E. Arch Dis Child. 1977 Mar;52(3):209–214. doi: 10.1136/adc.52.3.209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rachmilewitz D., Albers J. J., Saunders D. R. Apoprotein B in fasting and postprandial human jejunal mucosa. J Clin Invest. 1976 Feb;57(2):530–533. doi: 10.1172/JCI108307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Redgrave T. G., Roberts D. C., West C. E. Separation of plasma lipoproteins by density-gradient ultracentrifugation. Anal Biochem. 1975 May 12;65(1-2):42–49. doi: 10.1016/0003-2697(75)90488-1. [DOI] [PubMed] [Google Scholar]
  22. Schonfeld G., Bell E., Alpers D. H. Intestinal apoproteins during fat absorption. J Clin Invest. 1978 Jun;61(6):1539–1550. doi: 10.1172/JCI109074. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shulman M., Wilde C. D., Köhler G. A better cell line for making hybridomas secreting specific antibodies. Nature. 1978 Nov 16;276(5685):269–270. doi: 10.1038/276269a0. [DOI] [PubMed] [Google Scholar]
  24. Slot J. W., Geuze H. J. A new method of preparing gold probes for multiple-labeling cytochemistry. Eur J Cell Biol. 1985 Jul;38(1):87–93. [PubMed] [Google Scholar]
  25. Slot J. W., Geuze H. J. Sizing of protein A-colloidal gold probes for immunoelectron microscopy. J Cell Biol. 1981 Aug;90(2):533–536. doi: 10.1083/jcb.90.2.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Spencer K., Price C. P. Kinetic immunoturbidimetry: the estimation of albumin. Clin Chim Acta. 1979 Jul 16;95(2):263–276. doi: 10.1016/0009-8981(79)90368-1. [DOI] [PubMed] [Google Scholar]
  27. Steinberg D., Grundy S. M., Mok H. Y., Turner J. D., Weinstein D. B., Brown W. V., Albers J. J. Metabolic studies in an unusual case of asymptomatic familial hypobetalipoproteinemia with hypolphalipoproteinemia and fasting chylomicronemia. J Clin Invest. 1979 Jul;64(1):292–301. doi: 10.1172/JCI109451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Takashima Y., Kodama T., Iida H., Kawamura M., Aburatani H., Itakura H., Akanuma Y., Takaku F., Kawade M. Normotriglyceridemic abetalipoproteinemia in infancy: an isolated apolipoprotein B-100 deficiency. Pediatrics. 1985 Mar;75(3):541–546. [PubMed] [Google Scholar]
  29. Tokuyasu K. T. A study of positive staining of ultrathin frozen sections. J Ultrastruct Res. 1978 Jun;63(3):287–307. doi: 10.1016/s0022-5320(78)80053-7. [DOI] [PubMed] [Google Scholar]
  30. Tokuyasu K. T., Singer S. J. Improved procedures for immunoferritin labeling of ultrathin frozen sections. J Cell Biol. 1976 Dec;71(3):894–906. doi: 10.1083/jcb.71.3.894. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Windmueller H. G., Spaeth A. E. Regulated biosynthesis and divergent metabolism of three forms of hepatic apolipoprotein B in the rat. J Lipid Res. 1985 Jan;26(1):70–81. [PubMed] [Google Scholar]
  32. Wu A. L., Windmueller H. G. Variant forms of plasma apolipoprotein B. Hepatic and intestinal biosynthesis and heterogeneous metabolism in the rat. J Biol Chem. 1981 Apr 25;256(8):3615–3618. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES