Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1987 Feb;79(2):335–343. doi: 10.1172/JCI112817

Beta-adrenergic agonists increase lung liquid clearance in anesthetized sheep.

Y Berthiaume, N C Staub, M A Matthay
PMCID: PMC424066  PMID: 2879851

Abstract

We did experiments to determine whether beta-adrenergic agonists increase lung liquid clearance in anesthetized ventilated adult sheep and, if so, whether the increase is mediated by beta receptors and what mechanism is involved. We instilled 100 ml of autologous serum either alone or with a beta-adrenergic agonist (terbutaline, 10(-5) M, or epinephrine, 5.5 X 10(-6) M) into one lower lobe. After 4 h both terbutaline and epinephrine increased lung liquid clearance. The increase in lung liquid clearance was inhibited when propranolol (a beta blocker) or amiloride (a sodium channel blocker) was added to the terbutaline. Increased clearance was not explained by changes in pulmonary hemodynamics, pulmonary blood flow, or lung lymph flow. We conclude that beta-adrenergic agonists increase lung liquid clearance in anesthetized intact adult sheep. This increase is mediated through beta receptors and probably depends on increased active transport of sodium across the alveolar barrier.

Full text

PDF
340

Images in this article

337Image
on p.337
339Image
on p.339

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bland R. D., Boyd C. A. Cation transport in lung epithelial cells derived from fetal, newborn, and adult rabbits. J Appl Physiol (1985) 1986 Aug;61(2):507–515. doi: 10.1152/jappl.1986.61.2.507. [DOI] [PubMed] [Google Scholar]
  2. Carstairs J. R., Nimmo A. J., Barnes P. J. Autoradiographic visualization of beta-adrenoceptor subtypes in human lung. Am Rev Respir Dis. 1985 Sep;132(3):541–547. doi: 10.1164/arrd.1985.132.3.541. [DOI] [PubMed] [Google Scholar]
  3. Coates G., O'Brodovich H., Jefferies A. L., Gray G. W. Effects of exercise on lung lymph flow in sheep and goats during normoxia and hypoxia. J Clin Invest. 1984 Jul;74(1):133–141. doi: 10.1172/JCI111393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cotton C. U., Lawson E. E., Boucher R. C., Gatzy J. T. Bioelectric properties and ion transport of airways excised from adult and fetal sheep. J Appl Physiol Respir Environ Exerc Physiol. 1983 Nov;55(5):1542–1549. doi: 10.1152/jappl.1983.55.5.1542. [DOI] [PubMed] [Google Scholar]
  5. Crandall E. D., Heming T. A., Palombo R. L., Goodman B. E. Effects of terbutaline on sodium transport in isolated perfused rat lung. J Appl Physiol (1985) 1986 Jan;60(1):289–294. doi: 10.1152/jappl.1986.60.1.289. [DOI] [PubMed] [Google Scholar]
  6. Egan E. A., Nelson R. M., Gessner I. H. Solute permeability of the alveolar epithelium in acute hemodynamic pulmonary edema in dogs. Am J Physiol. 1977 Jul;233(1):H80–H86. doi: 10.1152/ajpheart.1977.233.1.H80. [DOI] [PubMed] [Google Scholar]
  7. Freedman F. B., Johnson J. A. Equilibrium and kinetic properties of the Evans blue-albumin system. Am J Physiol. 1969 Mar;216(3):675–681. doi: 10.1152/ajplegacy.1969.216.3.675. [DOI] [PubMed] [Google Scholar]
  8. Goodman B. E., Brown S. E., Crandall E. D. Regulation of transport across pulmonary alveolar epithelial cell monolayers. J Appl Physiol Respir Environ Exerc Physiol. 1984 Sep;57(3):703–710. doi: 10.1152/jappl.1984.57.3.703. [DOI] [PubMed] [Google Scholar]
  9. Goodman B. E., Crandall E. D. Dome formation in primary cultured monolayers of alveolar epithelial cells. Am J Physiol. 1982 Jul;243(1):C96–100. doi: 10.1152/ajpcell.1982.243.1.C96. [DOI] [PubMed] [Google Scholar]
  10. Goodman B. E., Fleischer R. S., Crandall E. D. Evidence for active Na+ transport by cultured monolayers of pulmonary alveolar epithelial cells. Am J Physiol. 1983 Jul;245(1):C78–C83. doi: 10.1152/ajpcell.1983.245.1.C78. [DOI] [PubMed] [Google Scholar]
  11. Heymann M. A., Payne B. D., Hoffman J. I., Rudolph A. M. Blood flow measurements with radionuclide-labeled particles. Prog Cardiovasc Dis. 1977 Jul-Aug;20(1):55–79. doi: 10.1016/s0033-0620(77)80005-4. [DOI] [PubMed] [Google Scholar]
  12. Kwong M. S., Egan E. A. Validation of [57Co]cyanocobalamin as an extracellular fluid marker and measurement of albumin exclusion from the interstitium in the rabbit. Microvasc Res. 1985 Mar;29(2):152–160. doi: 10.1016/0026-2862(85)90013-5. [DOI] [PubMed] [Google Scholar]
  13. Mason R. J., Williams M. C., Widdicombe J. H., Sanders M. J., Misfeldt D. S., Berry L. C., Jr Transepithelial transport by pulmonary alveolar type II cells in primary culture. Proc Natl Acad Sci U S A. 1982 Oct;79(19):6033–6037. doi: 10.1073/pnas.79.19.6033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Matthay M. A., Berthiaume Y., Staub N. C. Long-term clearance of liquid and protein from the lungs of unanesthetized sheep. J Appl Physiol (1985) 1985 Sep;59(3):928–934. doi: 10.1152/jappl.1985.59.3.928. [DOI] [PubMed] [Google Scholar]
  15. Matthay M. A., Landolt C. C., Staub N. C. Differential liquid and protein clearance from the alveoli of anesthetized sheep. J Appl Physiol Respir Environ Exerc Physiol. 1982 Jul;53(1):96–104. doi: 10.1152/jappl.1982.53.1.96. [DOI] [PubMed] [Google Scholar]
  16. Nelson R. M., McIntyre B. R., Egan E. A. Solute permeability of the alveolar epithelium in alloxan edema in dogs. J Appl Physiol Respir Environ Exerc Physiol. 1978 Mar;44(3):353–357. doi: 10.1152/jappl.1978.44.3.353. [DOI] [PubMed] [Google Scholar]
  17. Olver R. E. Fluid balance across the fetal alveolar epithelium. Am Rev Respir Dis. 1983 May;127(5 Pt 2):S33–S36. [PubMed] [Google Scholar]
  18. Olver R. E., Strang L. B. Ion fluxes across the pulmonary epithelium and the secretion of lung liquid in the foetal lamb. J Physiol. 1974 Sep;241(2):327–357. doi: 10.1113/jphysiol.1974.sp010659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Reddy N. P., Staub N. C. Intrinsic propulsive activity of thoracic duct perfused in anesthetized dogs. Microvasc Res. 1981 Mar;21(2):183–192. doi: 10.1016/0026-2862(81)90031-5. [DOI] [PubMed] [Google Scholar]
  20. Roos P. J., Wiener-Kronish J. P., Albertine K. H., Staub N. C. Removal of abdominal sources of caudal mediastinal node lymph in anesthetized sheep. J Appl Physiol Respir Environ Exerc Physiol. 1983 Sep;55(3):996–1001. doi: 10.1152/jappl.1983.55.3.996. [DOI] [PubMed] [Google Scholar]
  21. Selinger S. L., Bland R. D., Demling R. H., Staub N. C. Distribution volumes of [131I]albumin, [14C]sucrose, and 36Cl in sheep lung. J Appl Physiol. 1975 Nov;39(5):773–779. doi: 10.1152/jappl.1975.39.5.773. [DOI] [PubMed] [Google Scholar]
  22. Staub N. C. Alveolar flooding and clearance. Am Rev Respir Dis. 1983 May;127(5 Pt 2):S44–S51. [PubMed] [Google Scholar]
  23. Staub N. C., Bland R. D., Brigham K. L., Demling R., Erdmann A. J., 3rd, Woolverton W. C. Preparation of chronic lung lymph fistulas in sheep. J Surg Res. 1975 Nov;19(5):315–320. doi: 10.1016/0022-4804(75)90056-6. [DOI] [PubMed] [Google Scholar]
  24. Staub N. C., Schultz E. L., Koike K., Albertine K. H. Effect of neutrophil migration induced by leukotriene B4 on protein permeability in sheep lung. Fed Proc. 1985 Jan;44(1 Pt 1):30–35. [PubMed] [Google Scholar]
  25. Sugahara K., Caldwell J. H., Mason R. J. Electrical currents flow out of domes formed by cultured epithelial cells. J Cell Biol. 1984 Oct;99(4 Pt 1):1541–1544. doi: 10.1083/jcb.99.4.1541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. TAYLOR A. E., GUYTON A. C., BISHOP V. S. PERMEABILITY OF THE ALVEOLAR MEMBRANE TO SOLUTES. Circ Res. 1965 Apr;16:353–362. doi: 10.1161/01.res.16.4.353. [DOI] [PubMed] [Google Scholar]
  27. Walters D. V., Olver R. E. The role of catecholamines in lung liquid absorption at birth. Pediatr Res. 1978 Mar;12(3):239–242. doi: 10.1203/00006450-197803000-00017. [DOI] [PubMed] [Google Scholar]
  28. Widdicombe J. H., Welsh M. J. Ion transport by dog tracheal epithelium. Fed Proc. 1980 Nov;39(13):3062–3066. [PubMed] [Google Scholar]
  29. Zwillich C. W., Matthay M., Potts D. E., Adler R., Hofeldt F., Weil J. V. Thyrotoxicosis: comparison of effects of thyroid ablation and beta-adrenergic blockade on metabolic rate and ventilatory control. J Clin Endocrinol Metab. 1978 Mar;46(3):491–500. doi: 10.1210/jcem-46-3-491. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES