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. 1987 Apr;79(4):1026–1030. doi: 10.1172/JCI112914

Apical membrane chloride/base exchange in the rat proximal convoluted tubule.

R J Alpern
PMCID: PMC424279  PMID: 3558814

Abstract

To examine whether Cl/base exchange is present on the apical membrane of the proximal convoluted tubule, cell pH was measured fluorometrically in the in vivo microperfused rat proximal tubule with (2',7')-bis(carboxyethyl)-(5,6)-carboxyfluorescein. The effect of luminal chloride addition was examined in tubules perfused symmetrically with chloride-free solutions. In the absence of inhibitors, luminal chloride addition did not affect cell pH. However, after inhibition of basolateral membrane anion transport with peritubular 4-acetamido-4'-isothiocyano-(2,2')-disulfonic-stilbene (to amplify effects of apical membrane transport on cell pH), luminal chloride addition caused a small cell acidification (delta pHi = 0.02). When 1 mM formate was added to the solutions, luminal chloride addition caused a larger change in cell pH (delta pHi = 0.06) that was inhibited by (4,4')-diisothiocyano-(2,2')-disulfonic-stilbene. This stimulation of Cl/base exchange was not seen with 1 mM acetate addition. These results demonstrate apical membrane Cl/base exchange, a significant fraction of which is dependent on the presence of formate and probably represents Cl/formate exchange.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akiba T., Alpern R. J., Eveloff J., Calamina J., Warnock D. G. Electrogenic sodium/bicarbonate cotransport in rabbit renal cortical basolateral membrane vesicles. J Clin Invest. 1986 Dec;78(6):1472–1478. doi: 10.1172/JCI112738. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alpern R. J. Bicarbonate-water interactions in the rat proximal convoluted tubule. An effect of volume flux on active proton secretion. J Gen Physiol. 1984 Nov;84(5):753–770. doi: 10.1085/jgp.84.5.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alpern R. J., Chambers M. Cell pH in the rat proximal convoluted tubule. Regulation by luminal and peritubular pH and sodium concentration. J Clin Invest. 1986 Aug;78(2):502–510. doi: 10.1172/JCI112602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Alpern R. J., Howlin K. J., Preisig P. A. Active and passive components of chloride transport in the rat proximal convoluted tubule. J Clin Invest. 1985 Oct;76(4):1360–1366. doi: 10.1172/JCI112111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Alpern R. J. Mechanism of basolateral membrane H+/OH-/HCO-3 transport in the rat proximal convoluted tubule. A sodium-coupled electrogenic process. J Gen Physiol. 1985 Nov;86(5):613–636. doi: 10.1085/jgp.86.5.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Baum M., Berry C. A. Evidence for neutral transcellular NaCl transport and neutral basolateral chloride exit in the rabbit proximal convoluted tubule. J Clin Invest. 1984 Jul;74(1):205–211. doi: 10.1172/JCI111403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Biagi B. A., Giebisch G. Temperature dependence of transepithelial potential in isolated perfused rabbit proximal tubules. Am J Physiol. 1979 Mar;236(3):F302–F310. doi: 10.1152/ajprenal.1979.236.3.F302. [DOI] [PubMed] [Google Scholar]
  8. Biagi B. A., Sohtell M. Electrophysiology of basolateral bicarbonate transport in the rabbit proximal tubule. Am J Physiol. 1986 Feb;250(2 Pt 2):F267–F272. doi: 10.1152/ajprenal.1986.250.2.F267. [DOI] [PubMed] [Google Scholar]
  9. Boron W. F., Boulpaep E. L. Intracellular pH regulation in the renal proximal tubule of the salamander. Basolateral HCO3- transport. J Gen Physiol. 1983 Jan;81(1):53–94. doi: 10.1085/jgp.81.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Brazy P. C., Gunn R. B. Furosemide inhibition of chloride transport in human red blood cells. J Gen Physiol. 1976 Dec;68(6):583–599. doi: 10.1085/jgp.68.6.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Burckhardt B. C., Sato K., Frömter E. Electrophysiological analysis of bicarbonate permeation across the peritubular cell membrane of rat kidney proximal tubule. I. Basic observations. Pflugers Arch. 1984 May;401(1):34–42. doi: 10.1007/BF00581530. [DOI] [PubMed] [Google Scholar]
  12. Burnham C., Munzesheimer C., Rabon E., Sachs G. Ion pathways in renal brush border membranes. Biochim Biophys Acta. 1982 Mar 8;685(3):260–272. doi: 10.1016/0005-2736(82)90066-9. [DOI] [PubMed] [Google Scholar]
  13. Cabantchik Z. I., Rothstein A. The nature of the membrane sites controlling anion permeability of human red blood cells as determined by studies with disulfonic stilbene derivatives. J Membr Biol. 1972 Dec 29;10(3):311–330. doi: 10.1007/BF01867863. [DOI] [PubMed] [Google Scholar]
  14. Cassano G., Stieger B., Murer H. Na/H- and Cl/OH-exchange in rat jejunal and rat proximal tubular brush border membrane vesicles. Studies with acridine orange. Pflugers Arch. 1984 Mar;400(3):309–317. doi: 10.1007/BF00581565. [DOI] [PubMed] [Google Scholar]
  15. Frömter E., Gessner K. Active transport potentials, membrane diffusion potentials and streaming potentials across rat kidney proximal tubule. Pflugers Arch. 1974;351(1):85–98. doi: 10.1007/BF00603513. [DOI] [PubMed] [Google Scholar]
  16. Grassl S. M., Aronson P. S. Na+/HCO3-co-transport in basolateral membrane vesicles isolated from rabbit renal cortex. J Biol Chem. 1986 Jul 5;261(19):8778–8783. [PubMed] [Google Scholar]
  17. Green R., Bishop J. H., Giebisch G. Ionic requirements of proximal tubular sodium transport. III. Selective luminal anion substitution. Am J Physiol. 1979 Mar;236(3):F268–F277. doi: 10.1152/ajprenal.1979.236.3.F268. [DOI] [PubMed] [Google Scholar]
  18. Howlin K. J., Alpern R. J., Berry C. A., Rector F. C., Jr Evidence for electroneutral sodium chloride transport in rat proximal convoluted tubule. Am J Physiol. 1986 Apr;250(4 Pt 2):F644–F648. doi: 10.1152/ajprenal.1986.250.4.F644. [DOI] [PubMed] [Google Scholar]
  19. Ives H. E., Chen P. Y., Verkman A. S. Mechanism of coupling between Cl- and OH- transport in renal brush-border membranes. Biochim Biophys Acta. 1986 Dec 1;863(1):91–100. doi: 10.1016/0005-2736(86)90390-1. [DOI] [PubMed] [Google Scholar]
  20. Jacobson H. R. Characteristics of volume reabsorption in rabbit superficial and juxtamedullary proximal convoluted tubules. J Clin Invest. 1979 Mar;63(3):410–418. doi: 10.1172/JCI109317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Karniski L. P., Aronson P. S. Chloride/formate exchange with formic acid recycling: a mechanism of active chloride transport across epithelial membranes. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6362–6365. doi: 10.1073/pnas.82.18.6362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lucci M. S., Warnock D. G. Effects of anion-transport inhibitors on NaCl reabsorption in the rat superficial proximal convoluted tubule. J Clin Invest. 1979 Aug;64(2):570–579. doi: 10.1172/JCI109495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Moolenaar W. H., Tsien R. Y., van der Saag P. T., de Laat S. W. Na+/H+ exchange and cytoplasmic pH in the action of growth factors in human fibroblasts. Nature. 1983 Aug 18;304(5927):645–648. doi: 10.1038/304645a0. [DOI] [PubMed] [Google Scholar]
  24. Rector F. C., Jr Sodium, bicarbonate, and chloride absorption by the proximal tubule. Am J Physiol. 1983 May;244(5):F461–F471. doi: 10.1152/ajprenal.1983.244.5.F461. [DOI] [PubMed] [Google Scholar]
  25. Rink T. J., Tsien R. Y., Pozzan T. Cytoplasmic pH and free Mg2+ in lymphocytes. J Cell Biol. 1982 Oct;95(1):189–196. doi: 10.1083/jcb.95.1.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sabolić I., Burckhardt G. Proton pathways in rat renal brush-border and basolateral membranes. Biochim Biophys Acta. 1983 Oct 12;734(2):210–220. doi: 10.1016/0005-2736(83)90119-0. [DOI] [PubMed] [Google Scholar]
  27. Schwartz G. J. Absence of Cl- -OH- or Cl- -HCO3- exchange in the rabbit renal proximal tubule. Am J Physiol. 1983 Oct;245(4):F462–F469. doi: 10.1152/ajprenal.1983.245.4.F462. [DOI] [PubMed] [Google Scholar]
  28. Seifter J. L., Knickelbein R., Aronson P. S. Absence of Cl-OH exchange and NaCl cotransport in rabbit renal microvillus membrane vesicles. Am J Physiol. 1984 Nov;247(5 Pt 2):F753–F759. doi: 10.1152/ajprenal.1984.247.5.F753. [DOI] [PubMed] [Google Scholar]
  29. Shiuan D., Weinstein S. W. Evidence for electroneutral chloride transport in rabbit renal cortical brush border membrane vesicles. Am J Physiol. 1984 Nov;247(5 Pt 2):F837–F847. doi: 10.1152/ajprenal.1984.247.5.F837. [DOI] [PubMed] [Google Scholar]
  30. Thomas J. A., Buchsbaum R. N., Zimniak A., Racker E. Intracellular pH measurements in Ehrlich ascites tumor cells utilizing spectroscopic probes generated in situ. Biochemistry. 1979 May 29;18(11):2210–2218. doi: 10.1021/bi00578a012. [DOI] [PubMed] [Google Scholar]
  31. Warnock D. G., Yee V. J. Chloride uptake by brush border membrane vesicles isolated from rabbit renal cortex. Coupling to proton gradients and K+ diffusion potentials. J Clin Invest. 1981 Jan;67(1):103–115. doi: 10.1172/JCI110002. [DOI] [PMC free article] [PubMed] [Google Scholar]

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