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. 1981 May;67(5):973–976. doi: 10.1104/pp.67.5.973

The Assimilation of Ureides in Shoot Tissues of Soybeans 1

1. CHANGES IN ALLANTOINASE ACTIVITY AND UREIDE CONTENTS OF LEAVES AND FRUITS

Richard J Thomas 1, Larry E Schrader 1
PMCID: PMC425812  PMID: 16661804

Abstract

The ureides, allantoin and allantoic acid, are major forms of N transported from nodules to shoots in soybeans (Merr.). Little is known about the occurrence, localization, or properties of the enzymes involved in the assimilation of ureides in shoot tissues. We have examined the capacity of the shoot tissues to assimilate allantoin via allantoinase (EC 3.5.2.5) during leaf and fruit development in nodulated soybeans. Specific activity of allantoinase in leaves peaked during pod formation and early seed filling. In developing fruits allantoinase activity in the seeds was 2 to 4 times that in the pods when expressed on a fresh weight or organ basis. In seeds, the embryos contained the highest specific allantoinase activity. Stems and petioles also had appreciable allantoinase activity. With development, peaks in the amounts of allantoic acid, but not allantoin, were measured in both leaves and fruits suggesting that the assimilation of allantoic acid may be a limiting factor in ureide assimilation. Highest amounts of ureides were measured in the pith and xylem of stem tissues and in developing pod walls.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  2. Duke S. H., Schrader L. E., Henson C. A., Servaites J. C., Vogelzang R. D., Pendleton J. W. Low root temperature effects on soybean nitrogen metabolism and photosynthesis. Plant Physiol. 1979 May;63(5):956–962. doi: 10.1104/pp.63.5.956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Feller U. K., Soong T. S., Hageman R. H. Leaf Proteolytic Activities and Senescence during Grain Development of Field-grown Corn (Zea mays L.). Plant Physiol. 1977 Feb;59(2):290–294. doi: 10.1104/pp.59.2.290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Franke W., Thiemann A., Remily C., Möchel L., Heye K. Zur Kenntnis ureidspaltender Enzyme. I. Soja-Allantoinase. Enzymologia. 1965 Nov 6;29(3):251–271. [PubMed] [Google Scholar]
  5. Herridge D. F., Atkins C. A., Pate J. S., Rainbird R. M. Allantoin and Allantoic Acid in the Nitrogen Economy of the Cowpea (Vigna unguiculata [L.] Walp.). Plant Physiol. 1978 Oct;62(4):495–498. doi: 10.1104/pp.62.4.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McClure P. R., Israel D. W. Transport of nitrogen in the xylem of soybean plants. Plant Physiol. 1979 Sep;64(3):411–416. doi: 10.1104/pp.64.3.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. St Angelo A. J., Ory R. L. Localization of allantoinase in glyoxysomes of germinating castor beans. Biochem Biophys Res Commun. 1970 Jul 27;40(2):290–296. doi: 10.1016/0006-291x(70)91008-9. [DOI] [PubMed] [Google Scholar]
  8. Streeter J. G. Allantoin and Allantoic Acid in Tissues and Stem Exudate from Field-grown Soybean Plants. Plant Physiol. 1979 Mar;63(3):478–480. doi: 10.1104/pp.63.3.478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Theimer R. R., Beevers H. Uricase and allantoinase in glyoxysomes. Plant Physiol. 1971 Feb;47(2):246–251. doi: 10.1104/pp.47.2.246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Vogels G. D., Van der Drift C. Differential analyses of glyoxylate derivatives. Anal Biochem. 1970 Jan;33(1):143–157. doi: 10.1016/0003-2697(70)90448-3. [DOI] [PubMed] [Google Scholar]

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