Abstract
Proanthocyanidins and their potential precursors have been analyzed by paper chromatography and C18 reversed phase columns with high performance liquid chromatography. Total proanthocyanidins on a dry weight basis in cell suspension cultures derived from seedlings of Douglas fir (Pseudotsuga menziesii) were equal to or greater than those found in mature needles of randomly selected outdoor-grown trees. The major monomer and dimer were catechin and epicatechin-catechin, respectively. Although only procyanidins were detectable in cell suspension cultures, mature needles of outdoor-grown trees contained prodelphinidins as well. Immature needles (flush growth) of the same trees contained only trace amounts of prodelphinidins. Eriodictyol-7-glucoside and dihydroquercetin-3′-glucoside were present in all tissues examined. The amount of eriodictyol-7-glucoside was strongly correlated with total proanthocyanidins in immature needles of flush growth (r = 0.89, p = 0.001). The most complex pattern of flavonoids was found in flush growth needles, which contained in addition to the above, naringenin-7-glucoside and five to six flavone glycosides. Chlorogenic acid was detected only in seedlings and in flush growth needles.
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Selected References
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- Creasy L. L., Swain T. Structure of condensed tannins. Nature. 1965 Oct 9;208(5006):151–153. doi: 10.1038/208151a0. [DOI] [PubMed] [Google Scholar]
- Douglas B. L. A Re-evaluation of Guedel's Stages of Anesthesia: With particular reference to the ambulatory dental general anesthetic patient. J Am Dent Soc Anesthesiol. 1958 Jan;5(1):11–14. [PMC free article] [PubMed] [Google Scholar]
- Stafford H. A., Lester H. H. Procyanidins (Condensed Tannins) in Green Cell Suspension Cultures of Douglas Fir Compared with Those in Strawberry and Avocado Leaves by Means of C(18)-Reversed-phase Chromatography. Plant Physiol. 1980 Dec;66(6):1085–1090. doi: 10.1104/pp.66.6.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zaprometov M. N., Grisebach H. Dihydrokaempferol as precursor for catechins in the tea plant. Z Naturforsch C. 1973 Mar-Apr;28(3):113–115. doi: 10.1515/znc-1973-3-402. [DOI] [PubMed] [Google Scholar]