Abstract
The effects of kaempferol on the oxidative and phosphorylative properties of plant mitochondria from potato tubers and etiolated mung bean (Phaseolus aureus Roxb.) hypocotyls were investigated. Kaempferol inhibited the state 3 oxidation rate of malate, NADH, and succinate, but was without effect on the ascorbate-tetramethyl p-phenylenediamine oxidation rate. The inhibition was almost the same whether the mitochondria were in state 3 or in an uncoupled state 3. When 180 micromolar kaempferol was added during state 4, the tight coupling of succinate or NADH oxidation was not released. The results obtained indicate that kaempferol inhibits the mitochondrial electron flow at, or just after, the flavoprotein site.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bendall D. S., Bonner W. D. Cyanide-insensitive Respiration in Plant Mitochondria. Plant Physiol. 1971 Feb;47(2):236–245. doi: 10.1104/pp.47.2.236. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Douce R., Christensen E. L., Bonner W. D., Jr Preparation of intaintact plant mitochondria. Biochim Biophys Acta. 1972 Aug 17;275(2):148–160. doi: 10.1016/0005-2728(72)90035-7. [DOI] [PubMed] [Google Scholar]
- Koeppe D. E., Miller R. J. Kaempferol inhibitions of corn mitochondrial phosphorylation. Plant Physiol. 1974 Sep;54(3):374–378. doi: 10.1104/pp.54.3.374. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lance C., Bonner W. D. The respiratory chain components of higher plant mitochondria. Plant Physiol. 1968 May;43(5):756–766. doi: 10.1104/pp.43.5.756. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neuburger M., Douce R. Effect of bicarbonate and oxaloacetate on malate oxidation by spinach leaf mitochondria. Biochim Biophys Acta. 1980 Feb 8;589(2):176–189. doi: 10.1016/0005-2728(80)90036-5. [DOI] [PubMed] [Google Scholar]
- Phillips M. L., Williams G. R. Anion transporters in plant mitochondria. Plant Physiol. 1973 Apr;51(4):667–670. doi: 10.1104/pp.51.4.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tobin A., Djerdjour B., Journet E., Neuburger M., Douce R. Effect of NAD on Malate Oxidation in Intact Plant Mitochondria. Plant Physiol. 1980 Aug;66(2):225–229. doi: 10.1104/pp.66.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilson R. H., Hanson J. B. The effect of respiratory inhibitors on NADH, succinate and malate oxidation in corn mitochondria. Plant Physiol. 1969 Sep;44(9):1335–1341. doi: 10.1104/pp.44.9.1335. [DOI] [PMC free article] [PubMed] [Google Scholar]