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. 1982 Apr;69(4):978–982. doi: 10.1104/pp.69.4.978

Involvement of a Primary Electrogenic Pump in the Mechanism for HCO3− Uptake by the Cyanobacterium Anabaena variabilis1

Aaron Kaplan 1,2, Drora Zenvirth 1,2, Leonora Reinhold 1,2, Joseph A Berry 1,2
PMCID: PMC426339  PMID: 16662330

Abstract

The response of the membrane potential to HCO3− supply has been studied in the cyanobacterium Anabaena variabilis strain M-3 under various conditions. Changes in potential were followed with the aid of the lipophilic cation tetraphenyl phosphonium bromide.

Addition of HCO3− to CO2-depleted cells resulted in rapid hyperpolarization. The rate and extent of hyperpolarization were greater in low-CO2-adapted than in high-CO2-adapted cells. Addition of the electron acceptor p-nitrosodimethylaniline which resulted in O2 evolution in CO2-depleted cells did not cause hyperpolarization. The hyperpolarization was not attributable to a change in pH or in ionic strength of the medium. Pretreatment with 3-(3,4-dichlorophenyl)-1,1-dimethylurea prevented the hyperpolarization. KCN depolarized hyperpolarized cells. Addition of HCO3− also brought about immediate K+ influx which was succeeded after about 2 minutes by K+ efflux.

Two of the models considered would be capable of explaining these and previous findings: (a) a primary electrogenic pump for transporting HCO3− ions; (b) proton-HCO3− contransport, the driving force for which is generated by a proton pump which is sensitive to the HCO3− concentration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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