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. 1972 Feb;69(2):374–377. doi: 10.1073/pnas.69.2.374

Stimulation of DNA and Protein Synthesis in Fetal-Rat Liver Cells by Serum from Partially Hepatectomized Rats

Dieter Paul *, Hyam Leffert †, Gordon Sato ‡, Robert W Holley *
PMCID: PMC426461  PMID: 4501120

Abstract

Primary cultures of differentiated liver cells from fetal rats respond to serum from partially hepatectomized rats by an increased uptake of [3H]thymidine and [3H]leucine as compared with cultures incubated with normal rat serum. These findings are discussed in relation to the mechanism of induction of DNA synthesis and growth in liver cells during liver regeneration.

Keywords: primary culture, serum factors, proteins, liver regeneration, control mechanism

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUCHER N. L. REGENERATION OF MAMMALIAN LIVER. Int Rev Cytol. 1963;15:245–300. doi: 10.1016/s0074-7696(08)61119-5. [DOI] [PubMed] [Google Scholar]
  2. BUCHER N. L., SWAFFIELD M. N., DITROIA J. F. THE INFLUENCE OF AGE UPON THE INCORPORATION OF THYMIDINE-2-C14 INTO THE DNA OF REGENERATING RAT LIVER. Cancer Res. 1964 Apr;24:509–512. [PubMed] [Google Scholar]
  3. BUCHER N. L., SWAFFIELD M. N. THE RATE OF INCORPORATION OF LABELED THYMIDINE INTO THE DEOXYRIBONUCLEIC ACID OF REGENERATING RAT LIVER IN RELATION TO THE AMOUNT OF LIVER EXCISED. Cancer Res. 1964 Oct;24:1611–1625. [PubMed] [Google Scholar]
  4. Bucher N. L. Experimental aspects of hepatic regeneration. N Engl J Med. 1967 Sep 28;277(13):686–contd. doi: 10.1056/NEJM196709282771306. [DOI] [PubMed] [Google Scholar]
  5. Bucher N. L. Experimental aspects of hepatic regeneration. N Engl J Med. 1967 Oct 5;277(14):738–concl. doi: 10.1056/NEJM196710052771405. [DOI] [PubMed] [Google Scholar]
  6. CATER D. B., HOLMES B. E., MEE L. K. The effect of growth hormone upon cell division and nucleic acid synthesis in the regenerating liver of the rat. Biochem J. 1957 Jul;66(3):482–486. doi: 10.1042/bj0660482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FEIGELSON M., GROSS P. R., FEIGELSON P. Early effects of cortisone on nucleic acid and protein metabolism of rat liver. Biochim Biophys Acta. 1962 Apr 2;55:495–504. doi: 10.1016/0006-3002(62)90982-4. [DOI] [PubMed] [Google Scholar]
  8. FUJIOKA M., KOGA M., LIEBERMAN I. METABOLISM OF RIBONUCLEIC ACID AFTER PARTIAL HEPATECTOMY. J Biol Chem. 1963 Oct;238:3401–3406. [PubMed] [Google Scholar]
  9. Fisher B., Szuch P., Levine M., Fisher E. R. A portal blood factor as the humoral agent in liver regeneration. Science. 1971 Feb 12;171(3971):575–577. doi: 10.1126/science.171.3971.575. [DOI] [PubMed] [Google Scholar]
  10. Grisham J. W., Leong G. F., Albright M. L., Emerson J. D. Effect of exchange transfusion on labeling of nuclei with thymidine-3-H and on mitosis in hepatocytes of normal and regenerating rat liver. Cancer Res. 1966 Jul;26(7):1476–1485. [PubMed] [Google Scholar]
  11. HEMINGWAY J. T., CATER D. B. Effects of pituitary hormones and cortisone upon liver regeneration in the hypophysectomized rat. Nature. 1958 Apr 12;181(4615):1065–1066. doi: 10.1038/1811065a0. [DOI] [PubMed] [Google Scholar]
  12. HORVATH E., KOVACS K. Beiträge zur Rolle der Nebenniere in der Regeneration der Leber. Z Gesamte Exp Med. 1956;127(3):236–240. [PubMed] [Google Scholar]
  13. LEONG G. F., GRISHAM J. W., HOLE B. V., ALBRIGHT M. L. EFFECT OF PARTIAL HEPATECTOMY ON DNA SYNTHESIS AND MITOSIS IN HETEROTOPIC PARTIAL AUTOGRAFTS OF RAT LIVER. Cancer Res. 1964 Sep;24:1496–1501. [PubMed] [Google Scholar]
  14. LIEBERMAN I., SHORT J. HEPATIC BLOOD SUPPLY AND CONTROL OF DEOXYRIBONUCLEIC ACID SYNTHESIS IN LIVER. Am J Physiol. 1965 May;208:896–902. doi: 10.1152/ajplegacy.1965.208.5.896. [DOI] [PubMed] [Google Scholar]
  15. Moolten F. L., Bucher N. L. Regeneration of rat liver: transfer of humoral agent by cross circulation. Science. 1967 Oct 13;158(3798):272–274. doi: 10.1126/science.158.3798.272. [DOI] [PubMed] [Google Scholar]
  16. Moolten F. L., Oakman N. J., Bucher N. L. Accelerated response of hepatic DNA synthesis to partial hepatectomy in rats pretreated with growth hormone or surgical stress. Cancer Res. 1970 Sep;30(9):2353–2357. [PubMed] [Google Scholar]
  17. Nordenskjöld B. A., Skoog L., Brown N. C., Reichard P. Deoxyribonucleotide pools and deoxyribonucleic acid synthesis in cultured mouse embryo cells. J Biol Chem. 1970 Oct 25;245(20):5360–5368. [PubMed] [Google Scholar]
  18. Paul D., Lipton A., Klinger I. Serum factor requirements of normal and simian virus 40-transformed 3T3 mouse fibroplasts. Proc Natl Acad Sci U S A. 1971 Mar;68(3):645–652. doi: 10.1073/pnas.68.3.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rutzky L. P., Taylor W. G., Pumper R. W. In vitro stimulation of rat liver cells by homologous partial hepatectomy serum. In Vitro. 1971 Jul-Aug;7(1):1–5. doi: 10.1007/BF02618997. [DOI] [PubMed] [Google Scholar]
  20. SIGEL B., ACEVEDO F. J., DUNN M. R. The effect of patial hepatectomy on autotransplanted liver tissue. Surg Gynecol Obstet. 1963 Jul;117:29–36. [PubMed] [Google Scholar]
  21. Sakai A. Humoral factor triggering DNA synthesis after partial hepatectomy in the rat. Nature. 1970 Dec 19;228(5277):1186–1187. doi: 10.1038/2281186a0. [DOI] [PubMed] [Google Scholar]
  22. Sato G., Zaroff L., Mills S. E. TISSUE CULTURE POPULATIONS AND THEIR RELATION TO THE TISSUE OF ORIGIN. Proc Natl Acad Sci U S A. 1960 Jul;46(7):963–972. doi: 10.1073/pnas.46.7.963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Simek J., Erbenová Z., Deml F., Dvorácková I. Liver regeneration after partial hepatectomy in rats exposed before the operation to the stress stimulus. Experientia. 1968 Nov 15;24(11):1166–1167. doi: 10.1007/BF02147831. [DOI] [PubMed] [Google Scholar]
  24. Todaro G. J., Lazar G. K., Green H. The initiation of cell division in a contact-inhibited mammalian cell line. J Cell Physiol. 1965 Dec;66(3):325–333. doi: 10.1002/jcp.1030660310. [DOI] [PubMed] [Google Scholar]
  25. VAN LANCKER J. L., BORISON H. L. Incorporation of tritiumthymidine into rat-liver deoxyribonucleic acid after exchange transfusion with blood from partially-hepatectomized rats. Biochim Biophys Acta. 1961 Jul 22;51:171–172. doi: 10.1016/0006-3002(61)91030-7. [DOI] [PubMed] [Google Scholar]
  26. VIROLAINEN M. MITOTIC RESPONSE IN LIVER AUTOGRAFT AFTER PARTIAL HEPATECTOMY IN THE RAT. Exp Cell Res. 1964 Feb;33:588–591. doi: 10.1016/0014-4827(64)90027-8. [DOI] [PubMed] [Google Scholar]
  27. WINOCOUR E. Purification of polyoma virus. Virology. 1963 Feb;19:158–168. doi: 10.1016/0042-6822(63)90005-9. [DOI] [PubMed] [Google Scholar]
  28. WRBA H., RABES H. THE ACTION OF SERUM FROM PARTIALLY HEPATECTOMIZED RATS ON EXPLANTS OF LIVER AND TUMORS. Cancer Res. 1963 Sep;23:1116–1120. [PubMed] [Google Scholar]

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