Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Apr;69(4):872–876. doi: 10.1073/pnas.69.4.872

Glycolipid Synthesis in Normal and Virus-Transformed Hamster Cell Lines

Hisako Sakiyama 1, Sonja K Gross 1,*, Phillips W Robbins 1
PMCID: PMC426584  PMID: 4337244

Abstract

Studies have been continued on the synthesis of glycolipids by the NIL 2 line of hamster cells. Several clones were isolated from this line. These clones vary in morphology, saturation density, and glycolipid composition. Contrary to expectation there was no correlation between saturation density and complexity of the glycolipid pattern. In fact, the clone with the highest saturation density was the only one to show the complete set of glycolipids found previously in NIL 2 cells.

All untransformed NIL clones show an increase in the level of “higher” (more than two saccharides/ceramide) glycolipids as the cells approach confluence. In the line containing all three “higher” neutral glycolipids, all three increased as cells approached saturation density. In the line containing only hematoside, this compound increased at confluence. Cells transformed by hamster sarcoma virus or polyoma virus showed no density-dependent glycolipid synthesis.

Keywords: hamster sarcoma virus, polyoma virus, saturation density, Forssman antigen

Full text

PDF
873

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRADY R. O., KOVAL G. J. The enzymatic synthesis of sphingosine. J Biol Chem. 1958 Jul;233(1):26–31. [PubMed] [Google Scholar]
  2. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Diamond L. Two spontaneously transformed cell lines derived from the same hamster embryo culture. Int J Cancer. 1967 Mar 15;2(2):143–152. doi: 10.1002/ijc.2910020209. [DOI] [PubMed] [Google Scholar]
  4. Eckhart W. Complementation and transformation by temperature-sensitive mutants of polyoma virus. Virology. 1969 May;38(1):120–125. doi: 10.1016/0042-6822(69)90133-0. [DOI] [PubMed] [Google Scholar]
  5. Gray G. M. Chromatography of lipids. I. An improved chromatographic procedure for the quantitative isolation of the neutral ceramide-containing glycolipids from mammalian tissues. Biochim Biophys Acta. 1967 Dec 5;144(3):511–518. doi: 10.1016/0005-2760(67)90039-2. [DOI] [PubMed] [Google Scholar]
  6. Hakomori S. Cell density-dependent changes of glycolipid concentrations in fibroblasts, and loss of this response in virus-transformed cells. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1741–1747. doi: 10.1073/pnas.67.4.1741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. MACPHERSON I., MONTAGNIER L. AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS. Virology. 1964 Jun;23:291–294. doi: 10.1016/0042-6822(64)90301-0. [DOI] [PubMed] [Google Scholar]
  9. Mora P. T., Cumar F. A., Brady R. O. A common biochemical change in SV40 and polyoma virus transformed mouse cells coupled to control of cell growth in culture. Virology. 1971 Oct;46(1):60–72. doi: 10.1016/0042-6822(71)90006-7. [DOI] [PubMed] [Google Scholar]
  10. PUCK T. T., MARCUS P. I., CIECIURA S. J. Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer. J Exp Med. 1956 Feb 1;103(2):273–283. doi: 10.1084/jem.103.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. ROBINS E., LOWRY O. H., EYDT K. M., MCCAMAN R. E. Microdetermination of phospholipides and sphingolipides in brain. J Biol Chem. 1956 Jun;220(2):661–675. [PubMed] [Google Scholar]
  12. Robbins P. W., Macpherson I. A. Glycolipid synthesis in normal and transformed animal cells. Proc R Soc Lond B Biol Sci. 1971 Feb 16;177(1046):49–58. doi: 10.1098/rspb.1971.0014. [DOI] [PubMed] [Google Scholar]
  13. Robbins P. W., Macpherson I. Control of glycolipid synthesis in a cultured hamster cell line. Nature. 1971 Feb 19;229(5286):569–570. doi: 10.1038/229569a0. [DOI] [PubMed] [Google Scholar]
  14. Zavada J., Macpherson I. Transformation of hamster cell lines in vitro by a hamster sarcoma virus. Nature. 1970 Jan 3;225(5227):24–26. doi: 10.1038/225024a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES