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. 1972 May;69(5):1244–1248. doi: 10.1073/pnas.69.5.1244

Chemical Mutagenesis at the Phosphoribosyltransferase Locus in Cultured Human Lymphoblasts

Koki Sato 1,2,*, Ronald S Slesinski 1,2,†, John W Littlefield 1,2,‡
PMCID: PMC426673  PMID: 4504337

Abstract

The presence of selectable genetic markers in long-term human lymphoblast cultures would facilitate cell hybridization experiments on the biosynthesis of immunoglobulins, as well as other studies. This work reports the induction with ethylmethane sulfonate of 6-thioguanine - resistant, phosphoribosyltransferase - deficient mutants in a lymphoblast line from a patient with infectious mononucleosis. These cells were unusually sensitive, with a D0 value of 28 μg of ethylmethane sulfonate per ml; the sensitivity curve followed a biphasic pattern suggesting the presence of 3% resistant cells. Ethylmethane sulfonate increased the frequency of mutants resistant to 6-thioguanine over 100-fold, to about 2 × 10-4; nitrosoguanidine was less effective. Almost all the mutants contained considerably less than 1% of the hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) activity of wild-type cells. The mutation did not appear to result from loss of an X chromosome.

Keywords: ethylmethane sulfonate, 6-thioguanine resistance, Lesch-Nyhan syndrome

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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