Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Jun;69(6):1425–1428. doi: 10.1073/pnas.69.6.1425

Messenger RNA for Globin in the Postribosomal Supernatant of Rabbit Reticulocytes

M Jacobs-Lorena 1, C Baglioni 1
PMCID: PMC426717  PMID: 4504354

Abstract

10S RNA has been isolated from the postribosomal supernatant of rabbit reticulocyte lysate. This RNA is associated with protein in a ribonucleoprotein particle that sediments at approximately 20S. The 10S RNA extracted from this particle codes predominantly for α globin chains when it is translated by a mouse ascites cell-free extract. The product of the cell-free synthesis has been characterized by column chromatography and electrophoresis of the tryptic peptides. About 20% of the total α chain mRNA is present in the postribosomal supernatant, whereas β chain mRNA is almost exclusively associated with polyribosomes.

Keywords: mouse ascites tumor extract, α chain mRNA

Full text

PDF
1427

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baglioni C., La Via M., Ventruto V. A study of immunoglobulin structure. I. The fingerprinting of aminoethylated Bence-Jones proteins. Biochim Biophys Acta. 1965 Dec 16;111(2):479–484. doi: 10.1016/0304-4165(65)90057-7. [DOI] [PubMed] [Google Scholar]
  2. Baltimore D., Huang A. S. Interaction of HeLa cell proteins with RNA. J Mol Biol. 1970 Feb 14;47(3):263–273. doi: 10.1016/0022-2836(70)90301-3. [DOI] [PubMed] [Google Scholar]
  3. Colombo B., Baglioni C. Regulation of haemoglobin synthesis at the polysome level. J Mol Biol. 1966 Mar;16(1):51–66. doi: 10.1016/s0022-2836(66)80262-0. [DOI] [PubMed] [Google Scholar]
  4. DINTZIS H. M. Assembly of the peptide chains of hemoglobin. Proc Natl Acad Sci U S A. 1961 Mar 15;47:247–261. doi: 10.1073/pnas.47.3.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evans M. J., Lingrel J. B. Hemoglobin messenger ribonucleic acid. Synthesis of 9S and ribosomal ribonucleic acid during erythroid cell development. Biochemistry. 1969 Jul;8(7):3000–3005. doi: 10.1021/bi00835a048. [DOI] [PubMed] [Google Scholar]
  6. Housman D., Jacobs-Lorena M., Rajbhandary U. L., Lodish H. F. Initiation of haemoglobin synthesis by methionyl-tRNA. Nature. 1970 Aug 29;227(5261):913–918. doi: 10.1038/227913a0. [DOI] [PubMed] [Google Scholar]
  7. Housman D., Pemberton R., Taber R. Synthesis of and chains of rabbit hemoglobin in a cell-free extract from Krebs II ascites cells. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2716–2719. doi: 10.1073/pnas.68.11.2716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hunt R. T., Hunter A. R., Munro A. J. Control of haemoglobin synthesis: a difference in the size of the polysomes making alpha and beta chains. Nature. 1968 Nov 2;220(5166):481–483. doi: 10.1038/220481a0. [DOI] [PubMed] [Google Scholar]
  9. Lane C. D., Marbaix G., Gurdon J. B. Rabbit haemoglobin synthesis in frog cells: the translation of reticulocyte 9 s RNA in frog oocytes. J Mol Biol. 1971 Oct 14;61(1):73–91. doi: 10.1016/0022-2836(71)90207-5. [DOI] [PubMed] [Google Scholar]
  10. Lebleu B., Marbaix G., Huez G., Temmerman J., Burny A., Chantrenne H. Characterization of the messenger ribonucleoprotein released from reticulocyte polyribosomes by EDTA treatment. Eur J Biochem. 1971 Mar 11;19(2):264–269. doi: 10.1111/j.1432-1033.1971.tb01313.x. [DOI] [PubMed] [Google Scholar]
  11. Lockard R. E., Lingrel J. B. The synthesis of mouse hemoglobin beta-chains in a rabbit reticulocyte cell-free system programmed with mouse reticulocyte 9S RNA. Biochem Biophys Res Commun. 1969 Oct 8;37(2):204–212. doi: 10.1016/0006-291x(69)90720-7. [DOI] [PubMed] [Google Scholar]
  12. Mathews M. B., Osborn M., Lingrel J. B. Translation of globin messenger RNA in a heterologous cell-free system. Nature. 1971 Oct 13;233(5320):206–209. [PubMed] [Google Scholar]
  13. Nienhuis A. W., Laycock D. G., Anderson W. F. Translation of rabbit haemoglobin messenger RNA by thalassaemic and non-thalassaemic ribosomes. Nat New Biol. 1971 Jun 16;231(24):205–208. doi: 10.1038/newbio231205a0. [DOI] [PubMed] [Google Scholar]
  14. Schapira G., Padieu P., Maleknia N., Kruh J., Dreyfus J. C. Information génétique portée par une fraction soluble de réticulocytes et traduite en protéine spécifique sur les ribosomes d'une autre espèce. J Mol Biol. 1966 Oct;20(3):427–446. doi: 10.1016/0022-2836(66)90001-5. [DOI] [PubMed] [Google Scholar]
  15. Spirin A. S. The second Sir Hans Krebs Lecture. Informosomes. Eur J Biochem. 1969 Aug;10(1):20–35. doi: 10.1111/j.1432-1033.1969.tb00651.x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES