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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Jun;69(6):1508–1513. doi: 10.1073/pnas.69.6.1508

Measurement of Transmembrane Potentials in Phospholipid Vesicles

Roger D Kornberg 1,*, Mark G McNamee 1, Harden M McConnell 1,
PMCID: PMC426737  PMID: 4338594

Abstract

Phosphatidylcholine vesicles are permeable to tempotartrate, a spin-label derivative of tartaric acid. The inside-outside distribution of tempotartrate is coupled to the inside-outside distribution of H+, so it must be a measure of the transmembrane electrical potential difference in vesicles permeable to H+. This prediction is borne out by the finding that the inside-outside distribution of tempotartrate is the reciprocal of the inside-outside distribution of K+ in vesicles prepared in the presence of valinomycin.

The inside-outside distribution of tempotartrate is, by contrast, equal to the inside-outside distribution of Cl- in vesicles without valinomycin. This is evidence that an inside-outside Cl- concentration gradient induces an H+ gradient, which must be due to HCl permeation.

Keywords: spin labels, valinomycin

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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