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. 1972 Jun;69(6):1517–1520. doi: 10.1073/pnas.69.6.1517

Strand Orientation of Simian Virus 40 Transcription in Productively Infected Cells

Dona M Lindstrom 1, Renato Dulbecco 1
PMCID: PMC426739  PMID: 4338596

Abstract

Strand orientation of transcription in BSC-1 cells infected by simian virus 40 (SV40) was investigated by annealing RNAs extracted from infected cells with asymmetric complementary RNA from SV40 DNA synthesized in vitro by RNA polymerase from Escherichia coli. The results suggest that the early viral RNA sequences (those made before replication of viral DNA) and the late RNA sequences (those synthesized concurrently with replication of viral DNA) are transcribed from opposite strands of the SV40 DNA. The RNA synthesized in vitro is of the same polarity as the early in vivo RNA and is complementary to the late in vivo RNA. Knowledge of strand selection in lytic infection is a step towards understanding the regulation of transcription of this viral DNA.

Keywords: strand switch, mRNA, DNA virus

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aloni Y., Winocour E., Sachs L. Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells. J Mol Biol. 1968 Feb 14;31(3):415–429. doi: 10.1016/0022-2836(68)90418-x. [DOI] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burgess R. R. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6160–6167. [PubMed] [Google Scholar]
  4. Hatanaka M., Dulbecco R. Induction of DNA synthesis by SV40. Proc Natl Acad Sci U S A. 1966 Aug;56(2):736–740. doi: 10.1073/pnas.56.2.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  6. Martin M. A., Axelrod D. SV40 gene activity during lytic infection and in a series of SV40 transformed mouse cells. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1203–1210. doi: 10.1073/pnas.64.4.1203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Oda K., Dulbecco R. Regulation of transcription of the SV40 DNA in productively infected and in transformed cells. Proc Natl Acad Sci U S A. 1968 Jun;60(2):525–532. doi: 10.1073/pnas.60.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Oxman M. N., Levine A. S., Crumpacker C. S., Levin M. J., Henry P. H., Lewis A. M., Jr Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. IV. Characterization of the simian virus 40 ribonucleic acid species induced by wild-type simian virus 40 and by the nondefective hybrid virus, Ad2 + ND 1 . J Virol. 1971 Aug;8(2):215–224. doi: 10.1128/jvi.8.2.215-224.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sauer G. Apparent differences in transcriptional control in cells productively infected and transformed by SV40. Nat New Biol. 1971 Jun 2;231(22):135–138. doi: 10.1038/newbio231135a0. [DOI] [PubMed] [Google Scholar]
  10. Sauer G., Kidwai J. R. The transcription of the SV40 genome in productively infected and transformed cells. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1256–1263. doi: 10.1073/pnas.61.4.1256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Strauss J. H., Jr, Kelly R. B., Sinsheimer R. L. Denaturation of RNA with dimethyl sulfoxide. Biopolymers. 1968 Jun;6(6):793–807. doi: 10.1002/bip.1968.360060604. [DOI] [PubMed] [Google Scholar]
  12. VOGT M., DULBECCO R. Steps in the neoplastic transformation of hamster embryo cells by polyoma virus. Proc Natl Acad Sci U S A. 1963 Feb 15;49:171–179. doi: 10.1073/pnas.49.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Westphal H. SV40 DNA strand selection by Escherichia coli RNA polymerase. J Mol Biol. 1970 Jun 14;50(2):407–420. doi: 10.1016/0022-2836(70)90201-9. [DOI] [PubMed] [Google Scholar]
  14. Zimmerman S. B., Sandeen D. The ribonuclease activity of crystallized pancreatic deoxyribonuclease. Anal Biochem. 1966 Feb;14(2):269–277. doi: 10.1016/0003-2697(66)90137-0. [DOI] [PubMed] [Google Scholar]

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