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. 1972 Jun;69(6):1560–1564. doi: 10.1073/pnas.69.6.1560

RNA-Primed DNA Synthesis In Vitro

Walter Keller 1
PMCID: PMC426748  PMID: 4338598

Abstract

In vitro DNA synthesis on single-stranded circular DNA can be initiated by RNA primers. RNA chains are covalently extended by DNA polymerase II from KB cells and DNA polymerase I from Micrococcus luteus, but not by an RNA-dependent DNA polymerase from avian myeloblastosis virus. The reaction product consists of DNA chains with a piece of RNA at their 5′-ends, hydrogen bonded to the template DNA. The primer RNA is linked to the product DNA via a 3′:5′-phosphodiester bond, and can be specifically removed by ribonuclease H. The possible role of ribonuclease H in RNA-primed DNA synthesis in vivo is discussed.

Keywords: DNA polymerase, DNA initiation, RNA polymerase, ribonuclease H

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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