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. 1972 Jun;69(6):1578–1582. doi: 10.1073/pnas.69.6.1578

Adenovirus Messenger RNA in Mammalian Cells: Failure of Polyribosome Association in the Absence of Nuclear Cleavage

P M McGuire 1, C Swart 1, L D Hodge 1
PMCID: PMC426752  PMID: 4504372

Abstract

The nuclear synthesis of adenovirus-specific RNA late in the infectious cycle in the presence of toyocamycin (an adenosine analogue) has been investigated. There is reduced synthesis of viral RNA with an accumulation of virus-specific RNA in the molecular weight range of at least 4 to 8 × 106. No new viral RNA associates with cytoplasmic polyribosomes. In addition, hybridization competition experiments indicate a 70% competition between these large nuclear transcripts and polyribosome-associated viral RNA that was synthesized in the absence of inhibitor. These data are consistent with the following interpretations: complete nuclear processing of viral RNA is necessary for polyribosome association, and precursor viral message(s) contain sequences that are lost normally during post-transcriptional processing.

Keywords: toyocamycin, HeLa cells, viral transcripts

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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