Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Jun;69(6):1634–1637. doi: 10.1073/pnas.69.6.1634

Replication of Bacteriophage M 13: Inhibition of Single-Strand DNA Synthesis by Rifampicin

Walter L Staudenbauer 1, Peter Hans Hofschneider 1
PMCID: PMC426764  PMID: 4556466

Abstract

Synthesis of single-stranded DNA of bacteriophage M 13 is blocked by rifampicin. This inhibition is not observed in an Escherichia coli mutant possessing an RNA polymerase resistant to rifampicin. Since rifampicin stops single-strand synthesis faster than chloramphenicol, inhibition by rifampicin does not seem to result from a transcriptional block of protein synthesis, particularly in view of the long half-life of the messenger RNA for M 13. It is, therefore, concluded that the interaction of rifampicin with RNA polymerase directly affects single-strand DNA synthesis. Possible mechanisms are discussed.

Keywords: DNA replication, transcription, RNA polymerase, chloramphenicol, E. coli

Full text

PDF
1637

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brutlag D., Schekman R., Kornberg A. A possible role for RNA polymerase in the initiation of M13 DNA synthesis. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826–2829. doi: 10.1073/pnas.68.11.2826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burgess R. R. RNA polymerase. Annu Rev Biochem. 1971;40:711–740. doi: 10.1146/annurev.bi.40.070171.003431. [DOI] [PubMed] [Google Scholar]
  3. Forsheit A. B., Ray D. S. Replication of bacteriophage M13. VI. Attachment of M13 DNA to a fast-sedimenting host cell component. Virology. 1971 Mar;43(3):647–664. doi: 10.1016/0042-6822(71)90289-3. [DOI] [PubMed] [Google Scholar]
  4. Hohn B., Lechner H., Marvin D. A. Filamentous bacterial viruses. I. DNA synthesis during the early stages of infection with fd. J Mol Biol. 1971 Feb 28;56(1):143–154. doi: 10.1016/0022-2836(71)90090-8. [DOI] [PubMed] [Google Scholar]
  5. Jaenisch R., Jacob E., Hofschneider P. H. Replication of the small coliphage M13: evidence for long-living M13 specific messenger RNA. Nature. 1970 Jul 4;227(5253):59–60. doi: 10.1038/227059a0. [DOI] [PubMed] [Google Scholar]
  6. MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
  7. Marvin D. A., Hohn B. Filamentous bacterial viruses. Bacteriol Rev. 1969 Jun;33(2):172–209. doi: 10.1128/br.33.2.172-209.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pratt D., Erdahl W. S. Genetic control of bacteriophage M13 DNA synthesis. J Mol Biol. 1968 Oct 14;37(1):181–200. doi: 10.1016/0022-2836(68)90082-x. [DOI] [PubMed] [Google Scholar]
  9. Ray D. S. Replication of bacteriophage M13. II. The role of replicative forms in single-strand synthesis. J Mol Biol. 1969 Aug 14;43(3):631–643. doi: 10.1016/0022-2836(69)90364-7. [DOI] [PubMed] [Google Scholar]
  10. Ray D. S. Replication of bacteriophage M13. IV. Synthesis of M13-specific DNA in the presence of chloramphenicol. J Mol Biol. 1970 Oct 28;53(2):239–250. doi: 10.1016/0022-2836(70)90297-4. [DOI] [PubMed] [Google Scholar]
  11. Ray D. S., Schekman R. W. Replication of bacteriophage M13. I. Sedimentation analysis of crude lysates of M13-infected bacteria. Biochim Biophys Acta. 1969 Apr 22;179(2):398–407. [PubMed] [Google Scholar]
  12. Salstrom J. S., Pratt D. Role of coliphage M13 gene 5 in single-stranded DNA production. J Mol Biol. 1971 Nov 14;61(3):489–501. doi: 10.1016/0022-2836(71)90061-1. [DOI] [PubMed] [Google Scholar]
  13. Wehrli W., Staehelin M. Actions of the rifamycins. Bacteriol Rev. 1971 Sep;35(3):290–309. doi: 10.1128/br.35.3.290-309.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wirtz A., Hofschneider P. H. Replication of the single stranded DNA bacteriophage M 13. Intracellular flow of parental DNA and transfer to progeny particles. Eur J Biochem. 1970 Nov;17(1):141–150. doi: 10.1111/j.1432-1033.1970.tb01146.x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES