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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Jul;69(7):1716–1719. doi: 10.1073/pnas.69.7.1716

2′-O-Methylation of Adenosine, Guanosine, Uridine, and Cytidine in RNA of Isolated Rat Liver Nuclei

Adhid Al-Arif 1, Michael B Sporn 1,*
PMCID: PMC426785  PMID: 4340155

Abstract

Nuclei isolated from rat liver, when incubated with methyl-labeled S-adenosylmethionine, incorporated label into 2′-O-methyladenosine, 2′-O-methylguanosine, 2′-O-methyluridine, and 2′-O-methylcytidine of endogenous nuclear RNA. Addition of ribosomal RNA from wheat germ to the reaction markedly stimulated 2′-O-methylation of total RNA. The relative incorporation of label into the four different 2′-O-methyl ribonucleosides was greatly changed by the addition of wheat germ RNA. There was much more 2′-O-methylation of the purine ribonucleosides, relative to the pyrimidine ribonucleosides, in the reaction stimulated by wheat germ RNA.

Keywords: S-adenosyl methionine, wheat-germ RNA, paper chromatography

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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