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. 1972 Jul;69(7):1753–1757. doi: 10.1073/pnas.69.7.1753

Selective Inhibition of Nuclear DNA Synthesis by 9-β-D-Arabinofuranosyl Adenine in Rat Cells Transformed by Rous Sarcoma Virus

Charles Shipman Jr *, Sandra H Smith †, John C Drach ‡
PMCID: PMC426795  PMID: 4340157

Abstract

The drug, 9-β-D-arabinofuranosyl adenine, selectively inhibits the synthesis of nuclear DNA without affecting extrachromosomal DNA synthesis in rat cells transformed by Rous sarcoma virus (B-mix K-44/6). The inhibition was linear with respect to drug concentration over the range of 37-600 μM. Mitosis and total synthesis of DNA per cell were also depressed. DNA synthesis was determined by measurement of [3H]thymidine incorporation into DNA. Covalently-closed circular DNA was extracted by the Hirt procedure and separated from residual chromosomal DNA by buoyant density gradient ultracentrifugation in CsCl-propidium diiodide. On the basis of buoyant density and sedimentation velocity centrifugation, the covalently-closed circular DNA formed in the presence of the drug was indistinguishable from that formed in its absence.

Keywords: circular extrachromosomal DNA, CsCl-propidium diiodide centrifugation, rat embryo fibroblasts

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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