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. 1972 Jul;69(7):1835–1837. doi: 10.1073/pnas.69.7.1835

Coupling of a New, Active Morphine Derivative to Sepharose for Affinity Chromatography

Eric J Simon 1, William P Dole 1, Jacob M Hiller 1
PMCID: PMC426813  PMID: 4505662

Abstract

A new, pharmacologically active morphine derivative, 6-succinylmorphine, was synthesized. The properties of this compound and evidence for its structure are presented. Succinylmorphine was covalently coupled to ethylamino-Sepharose. Morphine-Sepharose containing up to 40 μg of morphine did not block the electrically stimulated contraction of isolated guinea pig ileum, but after alkaline hydrolysis of beads containing 2 μg of morphine the supernatant completely blocked contraction. This block was reversed by the specific morphine antagonist naloxone. Antibodies to morphine were removed from serum by morphine-Sepharose, but not by ethylamino-Sepharose, providing evidence of the efficacy of the beads for affinity chromatography.

Keywords: 6-succinylmorphine, opiate receptors, antibodies, naloxone

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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