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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Jul;69(7):1925–1928. doi: 10.1073/pnas.69.7.1925

Patterns of Simian Virus 40 DNA Transcription after Acute Infection of Permissive and Nonpermissive Cells*

George Khoury 1, Janet C Byrne 1, Malcolm A Martin 1
PMCID: PMC426833  PMID: 4340164

Abstract

Small amounts of fractionated, denatured, 32P-labeled DNA from SV40 virus were incubated with a large excess of the complementary RNA of SV40 prepared in vitro with Escherichia coli RNA polymerase; the viral DNA strands were separated on hydroxyapatite columns. The RNA present in green monkey cells late in the lytic cycle reacted with 40-42% of the strand complementary to the in vitro complementary RNA (minus strand), and 60-64% of the opposite (plus) strand. “Early lytic” RNA failed to significantly interact with the plus strand, but formed stable duplex molecules with 35-39% of the minus strand. The RNA prepared from mouse embryo cells 24 hr after infection with SV40 combined with 35-38% of the minus strand and 60-62% of the plus strand. In all cases, the same regions of either the plus or minus strand appear to be transcribed in permissive and nonpermissive infections.

Keywords: SV40, tissue culture, monkey, mouse, plus and minus strands, RNA synthesis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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