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. 1972 Jul;69(7):1943–1947. doi: 10.1073/pnas.69.7.1943

Membrane Dynamics in the Action of Dibutyryl Adenosine 3′:5′-Cyclic Monophosphate and Testosterone on Mammalian Cells

Theodore T Puck 1,2, Charles A Waldren 1,2, Abraham W Hsie 1,2
PMCID: PMC426837  PMID: 4340166

Abstract

The first manifestation of reverse transformation, in which a permanent epithelial-like cell is reversibly transformed into a fibroblast-like cell by dibutyryl adenosine cyclic 3′:5′-monophosphate, is the disappearance of a set of violently extending and retracting knobbed structures on the cell membrane with consequent tranquilization of the membrane. The cell then elongates to assume a fibroblast-like morphology. Permanently fibroblast-like cells, which have smooth membranes, can be changed into knobbed epithelial-like cells by the addition of either colcemid or cytochalasin B, agents that disorganize one or the other component, respectively, of the microtubular-microfibrillar system. Permanently smoothmembraned, epithelial-like cells also become knobbed by such treatment. All of these knobbed epithelial-like cells can be converted into smooth-membraned fibroblast-like cells by dibutyryl cAMP alone or with the aid of supporting molecules like testosterone. It is proposed that these are general phenomena and that the knobbed epithelial-like forms and the more highly differentiated smooth-membraned fibroblast-like cells represent extreme situations with respect to the degree of organization of the microtubular-microfibrillar system inside the cell.

Keywords: reverse transformation, epithelial-like cells, fibroblast-like cells, microtubular-microfibrillar system

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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