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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Jul;69(7):1972–1974. doi: 10.1073/pnas.69.7.1972

Complex Lipid Requirements for Detergent-Solubilized Phosphoacetylmuramyl-Pentapeptide Translocase from Micrococcus luteus

Jay N Umbreit 1, Jack L Strominger 1
PMCID: PMC426843  PMID: 4340168

Abstract

Phospho-MurNAc-pentapeptide translocase activity in the membrane of M. luteus was lost upon addition of the detergent, Triton X-100, but could be restored by addition of lipid fractions to the assay. By assay in the presence of lipid, the activity of the Triton-solubilized enzyme could be measured. The synthesis of C55-isoprenyl-P-P-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide required C55-isoprenyl-P, and was stimulated by a neutral lipid. The exchange reaction of UDP-MurNAc-pentapeptide with UMP required a polar lipid fraction, but the reaction was not affected by C55-isoprenyl-P or the neutral lipid. Thus, measurement of activity of the detergent-solubilized enzyme requires addition of three lipids, the lipid substrate (C55-isoprenyl-P), the neutral lipid, and a polar lipid.

Keywords: Triton X-100, cell wall synthesis, membrane-bound, C55-isoprenyl alcohol, enzyme

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1973

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON J. S., MATSUHASHI M., HASKIN M. A., STROMINGER J. L. LIPID-PHOSPHOACETYLMURAMYL-PENTAPEPTIDE AND LIPID-PHOSPHODISACCHARIDE-PENTAPEPTIDE: PRESUMED MEMBRANE TRANSPORT INTERMEDIATES IN CELL WALL SYNTHESIS. Proc Natl Acad Sci U S A. 1965 Apr;53:881–889. doi: 10.1073/pnas.53.4.881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  3. DAWSON R. M. A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples. Biochem J. 1960 Apr;75:45–53. doi: 10.1042/bj0750045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Heydanek M. G., Jr, Struve W. G., Neuhaus F. C. On the initial stage in peptidoglycan synthesis. 3. Kinetics and uncoupling of phospho-N-acetylmuramyl-pentapeptide translocase (uridine 5'-phosphate). Biochemistry. 1969 Mar;8(3):1214–1221. doi: 10.1021/bi00831a056. [DOI] [PubMed] [Google Scholar]

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