Abstract
Determination of quaternary structure by neutron scattering is proposed. The method gives the identity and relative spatial position of each component of a complex, provided that the complex can be obtained in deuterated form and can be reconstituted from its separated parts. If two components are rich in hydrogen and if the rest of the complex is deuterated, the contrast in scattering power permits the measurement of the separation of the pair from an interference cross term. The structure is obtained by triangulation from a set of measured pair separations.
Keywords: pair separations, triangulation, deuterated form, ribosomes, macromolecular aggregates
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Selected References
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- Hill W. E., Rossetti G. P., Van Holde K. E. Physical studies of ribosomes from Escherichia coli. J Mol Biol. 1969 Sep 14;44(2):263–277. doi: 10.1016/0022-2836(69)90174-0. [DOI] [PubMed] [Google Scholar]
- Hill W. E., Thompson J. D., Anderegg J. W. X-ray scattering study of ribosomes from Escherichia coli. J Mol Biol. 1969 Aug 28;44(1):89–102. doi: 10.1016/0022-2836(69)90406-9. [DOI] [PubMed] [Google Scholar]
- Maruta H., Tsuchiya T., Mizuno D. In vitro reassembly of functionally active 50 s ribosomal particles from ribosomal proteins and RNA's of Escherichia coli. J Mol Biol. 1971 Oct 14;61(1):123–134. doi: 10.1016/0022-2836(71)90210-5. [DOI] [PubMed] [Google Scholar]
- Nomura M., Erdmann V. A. Reconstitution of 50S ribosomal subunits from dissociated molecular components. Nature. 1970 Nov 21;228(5273):744–748. doi: 10.1038/228744a0. [DOI] [PubMed] [Google Scholar]
- SPAHR P. F. Amino acid composition of ribosomes from Escherichia coli. J Mol Biol. 1962 May;4:395–406. doi: 10.1016/s0022-2836(62)80020-5. [DOI] [PubMed] [Google Scholar]
- Schoenborn B. P. A neutron diffraction analysis of myoglobin. 3. Hydrogen-deuterium bonding in side chains. Cold Spring Harb Symp Quant Biol. 1972;36:569–575. doi: 10.1101/sqb.1972.036.01.071. [DOI] [PubMed] [Google Scholar]
- Traub P., Nomura M. Structure and function of E. coli ribosomes. V. Reconstitution of functionally active 30S ribosomal particles from RNA and proteins. Proc Natl Acad Sci U S A. 1968 Mar;59(3):777–784. doi: 10.1073/pnas.59.3.777. [DOI] [PMC free article] [PubMed] [Google Scholar]