Abstract
Interspecific plant hybrids have been produced by parasexual procedures. Protoplasts of Nicotiana glauca and N. langsdorffii were isolated, fused, and induced to regenerate into plants. The somatic hybrids were recovered from a mixed population of parental and fused protoplasts by a selective screening method that relies on differential growth of the hybrid on defined culture media. The biochemical and morphological characteristics of the somatically produced hybrid were identical to those of the sexually produced amphiploid.
Keywords: Nicotiana, leaf mesophyll, plant tissue culture, genetics, selective media
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- Carlson P. S. Induction and Isolation of Auxotrophic Mutants in Somatic Cell Cultures of Nicotiana tabacum. Science. 1970 Apr 24;168(3930):487–489. doi: 10.1126/science.168.3930.487. [DOI] [PubMed] [Google Scholar]
- Power J. B., Cummins S. E., Cocking E. C. Fusion of isolated plant protoplasts. Nature. 1970 Mar 14;225(5237):1016–1018. doi: 10.1038/2251016a0. [DOI] [PubMed] [Google Scholar]
- Schaeffer G. W., Smith H. H. Auxin-Kinetin Interaction in Tissue Cultures of Nicotiana Species & Tumor-Conditioned Hybrids. Plant Physiol. 1963 May;38(3):291–297. doi: 10.1104/pp.38.3.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith H. H. Plant genetic tumors. Prog Exp Tumor Res. 1972;15:138–164. [PubMed] [Google Scholar]