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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Aug;69(8):2312–2316. doi: 10.1073/pnas.69.8.2312

The Mode of Segregation of the Bacterial Cell Membrane

Elizabeth Weaver Green 1, Moselio Schaechter 1
PMCID: PMC426924  PMID: 4626404

Abstract

The distribution of phospholipids from labeled parental membranes to progeny cells was studied by autoradiography and a minicell system. The minicell experiments showed that, during growth, the parental membrane is diluted at the same rate in cells and in minicells, which indicates that ends of cells are not different from the cylindrical portions with regard to the distribution of parental molecules. The same result was obtained after labeling heme-containing proteins with δ-aminolevulinic acid. The autoradiographic experiments indicate that the membrane segregates in about 250 subunits 4 × 104 nm2 in size. These subunits appear to be conserved during growth.

Keywords: E. coli, minicell, phospholipids

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Selected References

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  1. Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beachey E. H., Cole R. M. Cell wall replication in Escherichia coli, studies by immunofluorescence and immunoelectron microscopy. J Bacteriol. 1966 Oct;92(4):1245–1251. doi: 10.1128/jb.92.4.1245-1251.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Briles E. B., Tomasz A. Radioautographic evidence for equatorial wall growth in a gram-positive bacterium. Segregation of choline-3H-labeled teichoic acid. J Cell Biol. 1970 Dec;47(3):786–790. doi: 10.1083/jcb.47.3.786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CHUNG K. L., HAWIRKO R. Z., ISAAC P. K. CELL WALL REPLICATION. I. CELL WALL GROWTH OF BACILLUS CEREUS AND BACILLUS MEGATERIUM. Can J Microbiol. 1964 Feb;10:43–48. doi: 10.1139/m64-007. [DOI] [PubMed] [Google Scholar]
  5. COLE R. M. CELL WALL REPLICATION IN SALMONELLA TYPHOSA. Science. 1964 Feb 21;143(3608):820–822. doi: 10.1126/science.143.3608.820. [DOI] [PubMed] [Google Scholar]
  6. COLE R. M., HAHN J. J. Cell wall replication in Streptococcus pyogenes. Science. 1962 Mar 2;135(3505):722–724. doi: 10.1126/science.135.3505.722. [DOI] [PubMed] [Google Scholar]
  7. Cole R. M. Symposium on the fine structure and replication of bacteria and their parts. 3. Bacterial cell-wall replication followed by immunofluorescence. Bacteriol Rev. 1965 Sep;29(3):326–344. doi: 10.1128/br.29.3.326-344.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Frehel C., Beaufils A. M., Ryter A. Etude au microscope électronique de la croissance de la paroi chez B. subtilis et B. megaterium. Ann Inst Pasteur (Paris) 1971 Aug;121(2):139–148. [PubMed] [Google Scholar]
  9. KANFER J., KENNEDY E. P. METABOLISM AND FUNCTION OF BACTERIAL LIPIDS. I. METABOLISM OF PHOSPHOLIPIDS IN ESCHERICHIA COLI B. J Biol Chem. 1963 Sep;238:2919–2922. [PubMed] [Google Scholar]
  10. Levy S. B., Leive L. Release from Escherichia coli of a galactosyltransferase complex active in lipopolysaccharide synthesis. J Biol Chem. 1970 Feb 10;245(3):585–594. [PubMed] [Google Scholar]
  11. Lin E. C., Hirota Y., Jacob F. On the process of cellular division in Escherichia coli. VI. Use of a methocel-autoradiographic method for the study of cellular division in Escherichia coli. J Bacteriol. 1971 Oct;108(1):375–385. doi: 10.1128/jb.108.1.375-385.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MAY J. W. Sites of cell-wall extension demonstrated by the use of fluorescent antibody. Exp Cell Res. 1962 Jun;27:170–172. doi: 10.1016/0014-4827(62)90060-5. [DOI] [PubMed] [Google Scholar]
  13. MacGregor C. H., Schnaitman C. A. Alterations in the cytoplasmic membrane proteins of various chlorate-resistant mutants of Escherichia coli. J Bacteriol. 1971 Oct;108(1):564–570. doi: 10.1128/jb.108.1.564-570.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mauck J., Chan L., Glaser L., Williamson J. Mode of cell wall growth of Bacillus megaterium. J Bacteriol. 1972 Jan;109(1):373–378. doi: 10.1128/jb.109.1.373-378.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. QUADLING C., STOCKER B. A. An environmentally-induced transition from the flagellated to the non-flagellated state in Salmonella typhimurium: the fate of parental flagella at cell division. J Gen Microbiol. 1962 Jun;28:257–270. doi: 10.1099/00221287-28-2-257. [DOI] [PubMed] [Google Scholar]
  16. Reeve J. N., Groves D. J., Clark D. J. Regulation of Cell Division in Escherichia coli: Characterization of Temperature-Sensitive Division Mutants. J Bacteriol. 1970 Dec;104(3):1052–1064. doi: 10.1128/jb.104.3.1052-1064.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schwarz U., Asmus A., Frank H. Autolytic enzymes and cell division of Escherichia coli. J Mol Biol. 1969 May 14;41(3):419–429. doi: 10.1016/0022-2836(69)90285-x. [DOI] [PubMed] [Google Scholar]
  18. Tsukagoshi N., Fielding P., Fox C. F. Membrane assembly in Escherichia coli. I. Segregation of preformed and newly formed membrane into daughter cells. Biochem Biophys Res Commun. 1971 Jul 16;44(2):497–502. doi: 10.1016/0006-291x(71)90629-2. [DOI] [PubMed] [Google Scholar]
  19. VAN TUBERGEN R. P., SETLOW R. B. Quantitative radioautographic studies on exponentially growing cultures of Escherichia coli. The distribution of parental DNA, RNA, protein, and cell wall among progeny cells. Biophys J. 1961 Sep;1:589–625. doi: 10.1016/s0006-3495(61)86911-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. White D. A., Lennarz W. J., Schnaitman C. A. Distribution of lipids in the wall and cytoplasmic membrane subfractions of the cell envelope of Escherichia coli. J Bacteriol. 1972 Feb;109(2):686–690. doi: 10.1128/jb.109.2.686-690.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wilson G., Fox C. F. Membrane assembly in Escherichia coli. II. Segregation of preformed and newly formed membrane proteins into cells and minicells. Biochem Biophys Res Commun. 1971 Jul 16;44(2):503–509. doi: 10.1016/0006-291x(71)90630-9. [DOI] [PubMed] [Google Scholar]

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