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. 1973 Oct;70(10):2921–2925. doi: 10.1073/pnas.70.10.2921

Biosynthesis of the Juvenile Hormones of Manduca sexta: Labeling Pattern from Mevalonate, Propionate, and Acetate

David A Schooley 1,*, Kenneth J Judy 1, B John Bergot 1, M Sharon Hall 1, John B Siddall 1
PMCID: PMC427139  PMID: 16592112

Abstract

Using organ culture, high-resolution liquid chromatography, and microchemical techniques, we demonstrated the efficient incorporation in vitro of several radiolabeled precursors into the two juvenile hormones of Manduca sexta. JH II, a homosesquiterpene hormone, reported from M. sexta as well as several other insects, incorporates radiolabel from acetate, mevalonate, and propionate. JH III, a sesquiterpene hormone recently reported as a natural product of M. sexta, incorporates label from acetate and mevalonate, but not from propionate. Based on the position of the labeled atoms in the precursors and upon the position of incorporation obtained from label-distribution data, a scheme for juvenile hormone biosynthesis is advanced.

Keywords: organ culture, corpora allata, high-resolution liquid chromatography, homomevalonate, homoterpenoid

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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