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. 1973 Nov;70(11):3111–3114. doi: 10.1073/pnas.70.11.3111

Cell-Surface Interactions: Differential Inhibition by Proflavine of Embryonic Cell Aggregation and Production of Specific Cell-Aggregating Factor

R E Hausman 1,2, A A Moscona 1,2
PMCID: PMC427181  PMID: 4522293

Abstract

Cell recognition and morphogenetic aggregation of embryonic cells into tissues are mediated by specific macromolecules in the cell surface (cell-aggregating factors). A factor specific for embryonic neural retina cells was demonstrated; its synthesis is required for histotypic reaggregation of retina cell suspensions. We show that proflavine (3,6-diaminoacridine) preferentially and reversibly suppresses production of the cell-aggregating factor and thereby inhibits normal cell reaggregation. If such proflavine-treated retina cells are exogenously supplied with the retina-specific factor, they reaggregate. The selectivity of these effects supports the postulated significance of specific cell-surface components in cell association into tissues; the results indicate that proflavine may be a useful molecular probe for studying formation of specific cell-surface components and their role in various cell interactions.

Keywords: cell ligands, cell recognition, differentiation, acridines

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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