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. 1973 Nov;70(11):3145–3149. doi: 10.1073/pnas.70.11.3145

Altered Enzymes in Drug-Resistant Variants of Mammalian Tissue Culture Cells

J D Sharp 1,*, N E Capecchi 1,*, M R Capecchi 1,*
PMCID: PMC427189  PMID: 4522295

Abstract

Two selective procedures are compared in an effort to isolate variants of mouse L cells containing structural gene mutations. Among the resulting variant cloned cell lines are found two types of alterations in the enzyme hypoxanthine phosphoribosyl transferase (EC 2.4.2.8) (1): enzyme with altered kinetic constants causing in vivo and in vitro resistance to 8-azaguanine; and (2) enzyme with altered heat sensitivity in vitro. These results support the view that tissue culture cell variants can arise from structural gene mutations.

Keywords: mouse L cells, somatic cell genetics, structural gene mutants, hypoxanthine phosphoribosyl transferase, 8-azaguanine resistance

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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