Abstract
We have isolated a mutant of E. coli whose maximal rate of synthesis of lac mRNA is 25-fold greater than that of its parental wild-type strain. The mutant also shows alterations in the kinetics of β-galactosidase synthesis, the functional half-life of β-galactosidase mRNA, and the properties of lac repressor.
Keywords: transcription, lac mRNA, competition hybridization
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bade E. G. Asymmetric transcription of bacteriophage Mu-1. J Virol. 1972 Dec;10(6):1205–1207. doi: 10.1128/jvi.10.6.1205-1207.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bauerle R. H., Margolin P. Evidence for two sites for initiation of gene expression in the tryptophan operon of Salmonella typhimurium. J Mol Biol. 1967 Jun 28;26(3):423–436. doi: 10.1016/0022-2836(67)90313-0. [DOI] [PubMed] [Google Scholar]
- Beckwith J., Grodzicker T., Arditti R. Evidence for two sites in the lac promoter region. J Mol Biol. 1972 Aug 14;69(1):155–160. doi: 10.1016/0022-2836(72)90031-9. [DOI] [PubMed] [Google Scholar]
- Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
- Friedman S. B., Margolin P. Evidence for an altered operator specificity: catabolite repression control of the leucine operon in Salmonella typhimurium. J Bacteriol. 1968 Jun;95(6):2263–2269. doi: 10.1128/jb.95.6.2263-2269.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KEPES A. KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA. Biochim Biophys Acta. 1963 Oct 15;76:293–309. [PubMed] [Google Scholar]
- Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MUNIER R., COHEN G. N. Incorporation d'analogues structuraux d'aminoacides dans les protéines bactériennes au cours de leur synthèse in vivo. Biochim Biophys Acta. 1959 Feb;31(2):378–391. doi: 10.1016/0006-3002(59)90011-3. [DOI] [PubMed] [Google Scholar]
- Michels C. A., Zipser D. The non-linear relationship between the enzyme activity and structural protein concentration of thiogalactoside transacetylase of E. coli. Biochem Biophys Res Commun. 1969 Feb 21;34(4):522–527. doi: 10.1016/0006-291x(69)90413-6. [DOI] [PubMed] [Google Scholar]
- Miller J. H., Beckwith J., Muller-Hill B. Direction of transcription of a regulatory gene in E. coli. Nature. 1968 Dec 28;220(5174):1287–1290. doi: 10.1038/2201287a0. [DOI] [PubMed] [Google Scholar]
- Müller-Hill B., Crapo L., Gilbert W. Mutants that make more lac repressor. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1259–1264. doi: 10.1073/pnas.59.4.1259. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scaife J., Beckwith J. R. Mutational alteration of the maximal level of Lac operon expression. Cold Spring Harb Symp Quant Biol. 1966;31:403–408. doi: 10.1101/sqb.1966.031.01.052. [DOI] [PubMed] [Google Scholar]
- Schwartz T., Craig E., Kennell D. Inactivation and degradation of messenger ribnucleic acid from the lactose operon of Escherichia coli. J Mol Biol. 1970 Dec 14;54(2):299–311. doi: 10.1016/0022-2836(70)90431-6. [DOI] [PubMed] [Google Scholar]
- Sharp P. A., Hsu M. T., Otsubo E., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol. 1972 Nov 14;71(2):471–497. doi: 10.1016/0022-2836(72)90363-4. [DOI] [PubMed] [Google Scholar]
- Silverstone A. E., Arditti R. R., Magasanik B. Catabolite-insensitive revertants of lac promoter mutants. Proc Natl Acad Sci U S A. 1970 Jul;66(3):773–779. doi: 10.1073/pnas.66.3.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith T. F., Sadler J. R. The nature of lactose operator constitive mutations. J Mol Biol. 1971 Jul 28;59(2):273–305. doi: 10.1016/0022-2836(71)90051-9. [DOI] [PubMed] [Google Scholar]
- Yu M. T., Vermeulen C. W., Atwood K. C. Location of the genes for 16S and 23S ribosomal RNA in the genetic map of Escherichia coli. Proc Natl Acad Sci U S A. 1970 Sep;67(1):26–31. doi: 10.1073/pnas.67.1.26. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zimmerman S. B., Sandeen D. The ribonuclease activity of crystallized pancreatic deoxyribonuclease. Anal Biochem. 1966 Feb;14(2):269–277. doi: 10.1016/0003-2697(66)90137-0. [DOI] [PubMed] [Google Scholar]