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. 1972 Jan;69(1):15–19. doi: 10.1073/pnas.69.1.15

Adenosine Triphosphatase Associated with Adenosine Triphosphate-Dependent Deoxyribonuclease

F G Nobrega 1,*, F H Rola 1,†, M Pasetto-Nobrega 1,*, M Oishi 1
PMCID: PMC427534  PMID: 4257817

Abstract

An ATPase activity that is completely dependent on DNA is associated with the ATP-dependent DNase (recB-recC enzyme) purified from Escherichia coli. There is a strong correlation between the ATPase and the DNase activities under various assay conditions. With E. coli DNA as substrate, 8-9 molecules of ATP are hydrolyzed to ADP and inorganic phosphate for every phosphodiester bond hydrolyzed by the DNase. The possible functional relationship of the ATPase and DNase activities is discussed.

Keywords: recB-recC enzyme, E. coli, ATP to phosphodiester hydrolysis ratio, DNA-dependent ATPase activity

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anai M., Hirahashi T., Yamanaka M., Takagi Y. A deoxyribonuclease which requires nucleoside triphosphate from Micrococcus lysodeikticus. II. Studies on the role of nucleoside triphosphate. J Biol Chem. 1970 Feb 25;245(4):775–780. [PubMed] [Google Scholar]
  2. Barbour S. D., Clark A. J. Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB+ and recC+ genes. Proc Natl Acad Sci U S A. 1970 Apr;65(4):955–961. doi: 10.1073/pnas.65.4.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Espejo R. T., Canelo E. S. The DNA of bacteriophage PM 2. Ultracentrifugal evidence for a circular structure. Virology. 1969 Mar;37(3):495–498. doi: 10.1016/0042-6822(69)90238-4. [DOI] [PubMed] [Google Scholar]
  4. GROSSMAN L., LEVINE S. S., ALLISON W. S. The reaction of formaldehyde with nucleotides and T2 bacteriophage DNA. J Mol Biol. 1961 Feb;3:47–60. doi: 10.1016/s0022-2836(61)80007-7. [DOI] [PubMed] [Google Scholar]
  5. Glynn I. M., Chappell J. B. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964 Jan;90(1):147–149. doi: 10.1042/bj0900147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldmark P. J., Linn S. An endonuclease activity from Escherichia coli absent from certain rec- strains. Proc Natl Acad Sci U S A. 1970 Sep;67(1):434–441. doi: 10.1073/pnas.67.1.434. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Howard-Flanders P., Boyce R. P. DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes. Radiat Res. 1966;(Suppl):156+–156+. [PubMed] [Google Scholar]
  8. Oishi M. An ATP-dependent deoxyribonuclease from Escherichia coli with a possible role in genetic recombination. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1292–1299. doi: 10.1073/pnas.64.4.1292. [DOI] [PMC free article] [PubMed] [Google Scholar]

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