Abstract
A series of 1,500 strains of Pseudomonas aeruginosa was collected from a variety of sources to provide a group of strains which would truly represent the species. All of them were pyocine typed, and a wide range of types was included among the isolates from each source. The gentamicin, carbenicillin, colistin, and polymyxin minimal inhibitory concentration of each strain was measured by the agar dilution method employing the Steers inocula replicator. Over 99% of strains were inhibited by 8 μg of gentamicin per ml, by 256 μg of carbenicillin per ml, and by 4 μg of colistin per ml. The small number of strains more resistant to each antibiotic comprised a variety of different pyocine types. Few strains were found to be susceptible to tetracycline, chloramphenicol, kanamycin, or streptomycin at the single concentration tested.
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- BARNETT M., BUSHBY S. R., WILKINSON S. SODIUM SULPHOMETHYL DERIVATIVES OF POLYMYXINS. Br J Pharmacol Chemother. 1964 Dec;23:552–574. doi: 10.1111/j.1476-5381.1964.tb01610.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bodey G. P., Terrell L. M. In vitro activity of carbenicillin against gram-negative bacilli. J Bacteriol. 1968 May;95(5):1587–1590. doi: 10.1128/jb.95.5.1587-1590.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Darrell J. H., Waterworth P. M. Dosage of gentamicin for pseudomonas infections. Br Med J. 1967 May 27;2(5551):535–537. doi: 10.1136/bmj.2.5551.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EICKHOFF T. C., FINLAND M. POLYMYXIN B AND COLISTIN: IN VITRO ACTIVITY AGAINST PSEUDOMONAS AERUGINOSA. Am J Med Sci. 1965 Feb;249:172–174. [PubMed] [Google Scholar]
- FEKETY F. R., Jr, NORMAN P. S., CLUFF L. E. The treatment of gram-negative bacillary infections with colistin. The toxicity and efficacy of large doses in forty-eight patients. Ann Intern Med. 1962 Aug;57:214–229. doi: 10.7326/0003-4819-57-2-214. [DOI] [PubMed] [Google Scholar]
- Garrod L. P., Waterworth P. M. Effect of medium composition on the apparent sensitivity of Pseudomonas aeruginosa to gentamicin. J Clin Pathol. 1969 Sep;22(5):534–538. doi: 10.1136/jcp.22.5.534. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gillies R. R., Govan J. R. Typing of Pseudomonas pyocyanea by pyocine production. J Pathol Bacteriol. 1966 Apr;91(2):339–345. doi: 10.1002/path.1700910207. [DOI] [PubMed] [Google Scholar]
- Govan J. R., Gillies R. R. Further studies in the pyocine typing of Pseudomonas pyocyanea. J Med Microbiol. 1969 Feb;2(1):17–25. doi: 10.1099/00222615-2-1-17. [DOI] [PubMed] [Google Scholar]
- Ingram L. C., Richmond M. H., Sykes R. B. Molecular characterization of the R factors implicated in the carbenicillin resistance of a sequence of Pseudomonas aeruginosa strains isolated from burns. Antimicrob Agents Chemother. 1973 Feb;3(2):279–288. doi: 10.1128/aac.3.2.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lowbury E. J., Babb J. R., Roe E. Clearance from a hospital of gram-negative bacilli that transfer carbenicillin-resistance to Pseudomonas aeruginosa. Lancet. 1972 Nov 4;2(7784):941–945. doi: 10.1016/s0140-6736(72)92469-5. [DOI] [PubMed] [Google Scholar]
- Lowbury E. J., Lilly H. A., Kidson A., Ayliffe G. A., Jones R. J. Sensitivity of Pseudomonas aeruginosa to antibiotics: emergence of strains highly resistant to carbenicillin. Lancet. 1969 Aug 30;2(7618):448–452. doi: 10.1016/s0140-6736(69)90163-9. [DOI] [PubMed] [Google Scholar]
- May J. R., Ingold A. Sensitivity of respiratory strains of Pseudomonas aeruginosa to carbenicillin. J Med Microbiol. 1973 Feb;6(1):77–82. doi: 10.1099/00222615-6-1-77. [DOI] [PubMed] [Google Scholar]
- Roe E., Jones R. J., Lowbury E. J. Transfer of anibioic resistanceetween Pseudomonas aeruginosa, Escherichia coli, and other gram-negative bacilli in rns. Lancet. 1971 Jan 23;1(7691):149–152. doi: 10.1016/s0140-6736(71)91930-1. [DOI] [PubMed] [Google Scholar]
- Rolinson G. N., Sutherland R. Carbenicillin, a new semisynthetic penicillin active against Pseudomonas aeruginosa. Antimicrob Agents Chemother (Bethesda) 1967;7:609–613. [PubMed] [Google Scholar]
- Shulman J. A., Terry P. M., Hough C. E. Colonization with gentamicin-resistant Pseudomonas aeruginosa, pyocine type 5, in a burn unit. J Infect Dis. 1971 Dec;124 (Suppl):S18–S23. doi: 10.1093/infdis/124.supplement_1.s18. [DOI] [PubMed] [Google Scholar]
- Traub W. H. Susceptibility of Pseudomonas aeruginosa to gentamicin sulfate in vitro: lack of correlation between disc diffusion and broth dilution sensitivity data. Appl Microbiol. 1970 Jul;20(1):98–102. doi: 10.1128/am.20.1.98-102.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Washington J. A., 2nd In vitro susceptibility of gram-negative bacilli to carbenicillin. Mayo Clin Proc. 1972 May;47(5):332–334. [PubMed] [Google Scholar]
- Weinstein M. J., Drube C. G., Moss E. L., Jr, Waitz J. A. Microbiologic studies related to bacterial resistance to gentamicin. J Infect Dis. 1971 Dec;124 (Suppl):S11–S17. doi: 10.1093/infdis/124.supplement_1.s11. [DOI] [PubMed] [Google Scholar]
- Witchitz J. L., Chabbert Y. A. Résistance transférable à la gentamicine I. Expression du caractère de résistance. Ann Inst Pasteur (Paris) 1971 Dec;121(6):733–742. [PubMed] [Google Scholar]
- Witchitz J. L., Chabbert Y. A. Résistance transférable à la gentamicine. II. Transmission et liaisons du caractère de résistance. Ann Inst Pasteur (Paris) 1972 Mar;122(3):367–378. [PubMed] [Google Scholar]