Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1974 Jun;5(6):565–570. doi: 10.1128/aac.5.6.565

Gentamicin-Adenylyltransferase Activity as a Cause of Gentamicin Resistance in Clinical Isolates of Pseudomonas aeruginosa

S Kabins 1, C Nathan 1, S Cohen 1
PMCID: PMC429015  PMID: 15825406

Abstract

Gentamicin adenylyltransferase activity was found in extracts of clinical isolates of gentamicin-resistant Pseudomonas aeruginosa. Extracts of one of these isolates, P. aeruginosa POW, inactivated gentamicin in the presence of adenosine 5′-triphosphate. Extracts of strain POW catalyzed the binding of radioactivity from [14C]adenine adenosine 5′-triphosphate to gentamicin components, tobramycin, sisomicin, kanamycin A and B and, to a variable degree, streptomycin and spectinomycin. The substrate profile with these agents and other aminocyclitols was similar to that obtained with R factor-mediated gentamicin adenylyltransferase found in Enterobacteriaceae. Adenylylating activity was absent in gentamicin-susceptible mutants of strain POW. Adenylylation may be added to acetylation as an enzymatic mechanism responsible for gentamicin resistance among strains of P. aeruginosa.

Full text

PDF
567

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. S., Lewis M. J. Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium. Nature. 1965 Nov 27;208(5013):843–849. doi: 10.1038/208843a0. [DOI] [PubMed] [Google Scholar]
  2. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  3. Benveniste R., Davies J. Mechanisms of antibiotic resistance in bacteria. Annu Rev Biochem. 1973;42:471–506. doi: 10.1146/annurev.bi.42.070173.002351. [DOI] [PubMed] [Google Scholar]
  4. Benveniste R., Davies J. R-factor mediated gentamicin resistance: A new enzyme which modifies aminoglycoside antibiotics. FEBS Lett. 1971 May 20;14(5):293–296. doi: 10.1016/0014-5793(71)80282-x. [DOI] [PubMed] [Google Scholar]
  5. Benveniste R., Yamada T., Davies J. Enzymatic Adenylylation of Streptomycin and Spectinomycin by R-Factor-Resistant Escherichia coli. Infect Immun. 1970 Jan;1(1):109–119. doi: 10.1128/iai.1.1.109-119.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bryan L. E., Semaka S. D., Van den Elzen H. M., Kinnear J. E., Whitehouse R. L. Characteristics of R931 and other Pseudomonas aeruginosa R factors. Antimicrob Agents Chemother. 1973 May;3(5):625–637. doi: 10.1128/aac.3.5.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Brzezinska M., Benveniste R., Davies J., Daniels P. J., Weinstein J. Gentamicin resistance in strains of Pseudomonas aeruginosa mediated by enzymatic N-acetylation of the deoxystreptamine moiety. Biochemistry. 1972 Feb 29;11(5):761–765. doi: 10.1021/bi00755a013. [DOI] [PubMed] [Google Scholar]
  8. Holloway B. W. Genetics of Pseudomonas. Bacteriol Rev. 1969 Sep;33(3):419–443. doi: 10.1128/br.33.3.419-443.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ingram L. C., Richmond M. H., Sykes R. B. Molecular characterization of the R factors implicated in the carbenicillin resistance of a sequence of Pseudomonas aeruginosa strains isolated from burns. Antimicrob Agents Chemother. 1973 Feb;3(2):279–288. doi: 10.1128/aac.3.2.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kabins S. A., Nathan C. R., Cohen S. R factor-mediated resistance to gentamicin in a clinical isolate of Escherichia coli. J Infect Dis. 1971 Dec;124 (Suppl):S65–S69. doi: 10.1093/infdis/124.supplement_1.s65. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Martin C. M., Ikari N. S., Zimmerman J., Waitz J. A. A virulent nosocomial Klebsiella with a transferable R factor for gentamicin: emergence and suppression. J Infect Dis. 1971 Dec;124 (Suppl):S24–S29. doi: 10.1093/infdis/124.supplement_1.s24. [DOI] [PubMed] [Google Scholar]
  13. Ozanne B., Benveniste R., Tipper D., Davies J. Aminoglycoside antibiotics: inactivation by phosphorylation in Escherichia coli carrying R factors. J Bacteriol. 1969 Nov;100(2):1144–1146. doi: 10.1128/jb.100.2.1144-1146.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Roe E., Jones R. J., Lowbury E. J. Transfer of anibioic resistanceetween Pseudomonas aeruginosa, Escherichia coli, and other gram-negative bacilli in rns. Lancet. 1971 Jan 23;1(7691):149–152. doi: 10.1016/s0140-6736(71)91930-1. [DOI] [PubMed] [Google Scholar]
  15. Sabath L. D., Casey J. I., Ruch P. A., Stumpf L. L., Finland M. Rapid microassay of gentamicin, kanamycin, neomycin, streptomycin, and vancomycin in serum or plasma. J Lab Clin Med. 1971 Sep;78(3):457–463. [PubMed] [Google Scholar]
  16. WATANABE T., FUKASAWA T. Episome-mediated transfer of drug resistance in Enterobacteriaceae. II. Elimination of resistance factors with acridine dyes. J Bacteriol. 1961 May;81:679–683. doi: 10.1128/jb.81.5.679-683.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Witchitz J. L., Chabbert Y. A. Résistance transférable à la gentamicine I. Expression du caractère de résistance. Ann Inst Pasteur (Paris) 1971 Dec;121(6):733–742. [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES