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. 1975 Dec;8(6):638–642. doi: 10.1128/aac.8.6.638

Presence of δ-(l-α-Aminoadipyl)-l-Cysteinyl-d-Valine in Fermentations of Penicillium chrysogenum

P Adriaens 1, B Meesschaert 1, W Wuyts 1, H Vanderhaeghe 1, H Eyssen 1
PMCID: PMC429440  PMID: 813571

Abstract

Cultures of Penicillium chrysogenum, growth with [35S]sulfate or labeled amino acids, were examined by ion-exchange chromatography for possible peptidic precursors of penicillin. A sulfur-containing compound, present in both the mycelial extracts and the culture filtrates, was eluted at the location of the synthetic lld-tripeptide δ-(l-α-aminoadipyl)-l-cysteinyl-d-valine. Since this compound was also labeled when the cultures were incubated with dl-[6-14C]α-aminoadipic acid, l-[3,3′-3H]cystine, or dl-[1-14C]valine, its identity with the synthetic lld-tripeptide can be accepted. No δ-(l-α-aminoadipyl)-l-cysteine or lll-tripeptide were detected. The implications of these findings for tripeptide and penicillin biosynthesis are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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