Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1976 Feb;9(2):239–246. doi: 10.1128/aac.9.2.239

Effect of Bleomycin on Deoxyribonucleic Acid Synthesis in Toluene-Treated Escherichia coli Cells

Steven L Ross 1, Robb E Moses 1
PMCID: PMC429510  PMID: 57740

Abstract

The antibiotic bleomycin stimulates deoxyribonucleic acid (DNA) synthesis in toluene-treated Escherichia coli cells. The increase in synthesis is linear with bleomycin concentration. Bleomycin-stimulated DNA synthesis is independent of replication and dependent on DNA polymerase I. Replication is spared as the DNA polymerase I-dependent DNA synthesis increases. Bleomycin does not appear to have any effect on purified E. coli DNA polymerases I or II. Our results suggest that bleomycin causes nicking of the bacterial chromosome with subsequent DNA synthesis catalyzed by DNA polymerase I.

Full text

PDF
246

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. APOSHIAN H. V., KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme. J Biol Chem. 1962 Feb;237:519–525. [PubMed] [Google Scholar]
  2. Buttin G., Wright M. Enzymatic DNA degradation in E. coli: its relationship to synthetic processes at the chromosome level. Cold Spring Harb Symp Quant Biol. 1968;33:259–269. doi: 10.1101/sqb.1968.033.01.030. [DOI] [PubMed] [Google Scholar]
  3. Campbell J. L., Shizuya H., Richardson C. C. Mapping of a mutation, polB100, affecting deoxyribonucleic acid polymerase II in Escherichia coli K-12. J Bacteriol. 1974 Aug;119(2):494–499. doi: 10.1128/jb.119.2.494-499.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
  5. Fujimura S., Makino T., Hayashi T. The effect of bleomycin on prophage induction and staphylokinase production. Jpn J Exp Med. 1972 Feb;42(1):75–80. [PubMed] [Google Scholar]
  6. Fujiwara Y., Kondo T. Strand-scission of HeLa cell deoxyribonucleic acid by bleomycin in vitro and in vivo. Biochem Pharmacol. 1973 Feb 1;22(3):323–333. doi: 10.1016/0006-2952(73)90413-9. [DOI] [PubMed] [Google Scholar]
  7. Gefter M. L., Hirota Y., Kornberg T., Wechsler J. A., Barnoux C. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150–3153. doi: 10.1073/pnas.68.12.3150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Haidle C. W. Fragmentation of deoxyribonucleic acid by bleomycin. Mol Pharmacol. 1971 Nov;7(6):645–652. [PubMed] [Google Scholar]
  9. Haidle C. W., Weiss K. K., Kuo M. T. Release of free bases from deoxyribonucleic acid after reaction with bleomycin. Mol Pharmacol. 1972 Sep;8(5):531–537. [PubMed] [Google Scholar]
  10. Haidle C. W., Weiss K. K., Mace M. L., Jr Induction of bacteriophage by bleomycin. Biochem Biophys Res Commun. 1972 Sep 5;48(5):1179–1184. doi: 10.1016/0006-291x(72)90835-2. [DOI] [PubMed] [Google Scholar]
  11. Hirota Y., Mordoh J., Scheffler I., Jacob F. Genetic approach to DNA replication and its control in Escherichia coli. Fed Proc. 1972 Sep-Oct;31(5):1422–1427. [PubMed] [Google Scholar]
  12. Jovin T. M., Englund P. T., Bertsch L. L. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase. J Biol Chem. 1969 Jun 10;244(11):2996–3008. [PubMed] [Google Scholar]
  13. Kelly R. B., Cozzarelli N. R., Deutscher M. P., Lehman I. R., Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. J Biol Chem. 1970 Jan 10;245(1):39–45. [PubMed] [Google Scholar]
  14. Konrad E. B., Lehman I. R. A conditional lethal mutant of Escherichia coli K12 defective in the 5' leads to 3' exonuclease associated with DNA polymerase I. Proc Natl Acad Sci U S A. 1974 May;71(5):2048–2051. doi: 10.1073/pnas.71.5.2048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kornberg T., Gefter M. L. Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II. Proc Natl Acad Sci U S A. 1971 Apr;68(4):761–764. doi: 10.1073/pnas.68.4.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Masker W. E., Hanawalt P. C. Ultraviolet-stimulated DNA synthesis in toluenzied Escherichia coli deficient in DNA polymerase I. Proc Natl Acad Sci U S A. 1973 Jan;70(1):129–133. doi: 10.1073/pnas.70.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moses R. E. Replicative deoixyribonucleic acid synthesis in a system diffusible for macromolecules. J Biol Chem. 1972 Oct 10;247(19):6031–6038. [PubMed] [Google Scholar]
  18. Moses R. E., Richardson C. C. A new DNA polymerase activity of Escherichia coli. I. Purification and properties of the activity present in E. coli polA1. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1557–1564. doi: 10.1016/0006-291x(70)90565-6. [DOI] [PubMed] [Google Scholar]
  19. Moses R. E., Richardson C. C. Replication and repair of DNA in cells of Escherichia coli treated with toluene. Proc Natl Acad Sci U S A. 1970 Oct;67(2):674–681. doi: 10.1073/pnas.67.2.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Müller W. E., Yamazaki Z. I., Zahn R. K. Bleomycin, a selective inhibitor of DNA-dependent DNA polymerase from oncogenic RNA viruses. Biochem Biophys Res Commun. 1972 Feb 25;46(4):1667–1673. doi: 10.1016/0006-291x(72)90801-7. [DOI] [PubMed] [Google Scholar]
  21. Müller W. E., Yamazaki Z., Breter H. J., Zahn R. K. Action of bleomycin on DNA and RNA. Eur J Biochem. 1972 Dec 18;31(3):518–525. doi: 10.1111/j.1432-1033.1972.tb02560.x. [DOI] [PubMed] [Google Scholar]
  22. RICHARDSON C. C., SCHILDKRAUT C. L., APOSHIAN H. V., KORNBERG A. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. J Biol Chem. 1964 Jan;239:222–232. [PubMed] [Google Scholar]
  23. Reiter H. ATP-independent, DNA synthesis by DNA polymerase II in toluenized Bacillus subtilis. Biochem Biophys Res Commun. 1974 Oct 23;60(4):1371–1377. doi: 10.1016/0006-291x(74)90349-0. [DOI] [PubMed] [Google Scholar]
  24. Suzuki H., Nagai K., Yamaki H., Tanaka N., Umezawa H. Mechanism of action of bleomycin. Studies with the growing culture of bacterial and tumor cells. J Antibiot (Tokyo) 1968 Jun;21(6):379–386. doi: 10.7164/antibiotics.21.379. [DOI] [PubMed] [Google Scholar]
  25. Suzuki H., Nagai K., Yamaki H., Tanaka N., Umezawa H. On the mechanism of action of bleomycin: scission of DNA strands in vitro and in vivo. J Antibiot (Tokyo) 1969 Sep;22(9):446–448. doi: 10.7164/antibiotics.22.446. [DOI] [PubMed] [Google Scholar]
  26. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Umezawa H., Asakura H., Oda K., Hori S., Hori M. The effect of bleomycin on SV40 DNA: characteristics of bleomycin action which produces a single scission in a superhelical form of SV40 DNA. J Antibiot (Tokyo) 1973 Sep;26(9):521–527. doi: 10.7164/antibiotics.26.521. [DOI] [PubMed] [Google Scholar]
  28. Umezawa H. Chemistry and mechanism of action of bleomycin. Fed Proc. 1974 Nov;33(11):2296–2302. [PubMed] [Google Scholar]
  29. Umezawa H., Maeda K., Takeuchi T., Okami Y. New antibiotics, bleomycin A and B. J Antibiot (Tokyo) 1966 Sep;19(5):200–209. [PubMed] [Google Scholar]
  30. Umezawa H., Suhara Y., Takita T., Maeda K. Purification of bleomycins. J Antibiot (Tokyo) 1966 Sep;19(5):210–215. [PubMed] [Google Scholar]
  31. Yagoda A., Mukherji B., Young C., Etcubanas E., Lamonte C., Smith J. R., Tan C. T., Krakoff I. H. Bleomycin, an antitumor antibiotic. Clinical experience in 274 patients. Ann Intern Med. 1972 Dec;77(6):861–870. doi: 10.7326/0003-4819-77-6-861. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES