Abstract
Urine from patients receiving metronidazole [1-(2-hydroxyethyl)-2-methyl-5-nitromidazole] orally or per vagina were subjected to paper chromatographic fractionation and examined for anticlostridial activity. Unmodified metronidazole and several metabolites were detected. These findings indicate that the clostridial bioassay may be limited in its applicability to the study of the pharmacodynamics of metronidazole as it does not differentiate between the parent substance and some of its metabolites. Moreover, some of the latter, although they possess antibacterial activity, may not have antiprotozoan activity.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- COSAR C., JULOU L. Activité de l'(hydroxy-2'éthyl)-1 méthyl-2 nitro-5 imidazole (8.823 R. P.) vis-à-vis des infections expérimentales à Trichomonas vaginalis. Ann Inst Pasteur (Paris) 1959 Feb;96(2):238–241. [PubMed] [Google Scholar]
- Everett E. D. Metronidazole and amebiasis. Am J Dig Dis. 1974 Jul;19(7):626–636. doi: 10.1007/BF01073018. [DOI] [PubMed] [Google Scholar]
- Long P. I. Cutaneous leishmaniasis treated with metronidazole. JAMA. 1973 Mar 19;223(12):1378–1379. [PubMed] [Google Scholar]
- Nastro L. J., Finegold S. M. Bactericidal activity of five antimicrobial agents against Bacteroides fragilis. J Infect Dis. 1972 Jul;126(1):104–107. doi: 10.1093/infdis/126.1.104. [DOI] [PubMed] [Google Scholar]
- Powell S. J., MacLeod I., Wilmot A. J., Elsdon-Dew R. Metronidazole in amoebic dysentery and amoebic liver abscess. Lancet. 1966 Dec 17;2(7477):1329–1331. doi: 10.1016/s0140-6736(66)92081-2. [DOI] [PubMed] [Google Scholar]
- Ralph E. D., Clarke J. T., Libke R. D., Luthy R. P., Kirby W. M. Pharmacokinetics of metronidazole as determined by bioassay. Antimicrob Agents Chemother. 1974 Dec;6(6):691–696. doi: 10.1128/aac.6.6.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stambaugh J. E., Feo L. G., Manthei R. W. Isolation and identification of the major urinary metabolite of metronidazole. Life Sci. 1967 Sep 1;6(17):1811–1819. doi: 10.1016/0024-3205(67)90209-3. [DOI] [PubMed] [Google Scholar]
- Stambaugh J. E., Feo L. G., Manthei R. W. The isolation and identification of the urinary oxidative metabolites of metronidazole in man. J Pharmacol Exp Ther. 1968 Jun;161(2):373–381. [PubMed] [Google Scholar]
- Tally F. P., Sutter V. L., Finegold S. M. Metronidazole versus anaerobes. In vitro data and initial clinical observations. Calif Med. 1972 Dec;117(6):22–26. [PMC free article] [PubMed] [Google Scholar]