Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1976 Jun;9(6):889–892. doi: 10.1128/aac.9.6.889

Characterization of an Ampicillin-Resistant Haemophilus influenzae Type B

Theodore C Eickhoff 1, Josephine M Ehret 1, R Dixie Baines 1
PMCID: PMC429645  PMID: 1084727

Abstract

A 28-year-old female in Denver was found in early 1974 to have frontal sinusitis, osteomyelitis, and bacteremia due to Haemophilus influenzae, type B. The minimal inhibitory concentration of ampicillin for this organism was 100 μg/ml and the minimal bactericidal concentration was >100 μg/ml. It was inhibited by chloramphenicol at 0.4 μg/ml. Further studies demonstrated that ampicillin and methicillin were synergistic against this organism. It was shown to produce a diffusible beta-lactamase. Transferase of resistance from this organism to a susceptible Haemophilus parainfluenzae and a reciprocal transfer were accomplished. A test for transformation was negative as was a test for reversal of resistance by ethylenediaminetetraacetic acid.

Full text

PDF
890

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALEXANDER H. E., LEIDY G., HAHN E. Studies on the nature of hemophilus influenzae cells susceptible to heritable changes by desoxyribonucleic acids. J Exp Med. 1954 Jun 1;99(6):505–533. doi: 10.1084/jem.99.6.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  3. Datta N., Kontomichalou P. Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae. Nature. 1965 Oct 16;208(5007):239–241. doi: 10.1038/208239a0. [DOI] [PubMed] [Google Scholar]
  4. Elwell L. P., De Graaff J., Seibert D., Falkow S. Plasmid-linked ampicillin resistance in haempohilus influenza type b. Infect Immun. 1975 Aug;12(2):404–410. doi: 10.1128/iai.12.2.404-410.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Farrar W. E., Jr, O'Dell N. M. Beta-lactamase activity in ampicillin-resistant Haemophilus influenzae. Antimicrob Agents Chemother. 1974 Nov;6(5):625–629. doi: 10.1128/aac.6.5.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Juni E. Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus. J Bacteriol. 1972 Nov;112(2):917–931. doi: 10.1128/jb.112.2.917-931.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Khan W., Ross S., Rodriguez W., Controni G., Saz A. K. Haemophilus influenzae type B resistant to ampicillin. A report of two cases. JAMA. 1974 Jul 15;229(3):298–301. [PubMed] [Google Scholar]
  8. Medeiros A. A., O'Brien T. F. Ampicillin-resistant Haemophilus influenzae type B possessing a TEM-type beta-lactamase but little permeability barrier to ampicillin. Lancet. 1975 Mar 29;1(7909):716–719. doi: 10.1016/s0140-6736(75)91630-x. [DOI] [PubMed] [Google Scholar]
  9. Neu H. C. Effect of beta-lactamase location in Escherichia coli on penicillin synergy. Appl Microbiol. 1969 Jun;17(6):783–786. doi: 10.1128/am.17.6.783-786.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Smith D. H., Armour S. E. Transferable R factors in enteric bacteria causing infection of the genitourinary tract. Lancet. 1966 Jul 2;2(7453):15–18. doi: 10.1016/s0140-6736(66)91745-4. [DOI] [PubMed] [Google Scholar]
  11. Sykes R. B., Matthew M., O'Callaghan C. H. R-factor mediated beta-lactamase production by Haemophilus influenzae. J Med Microbiol. 1975 Aug;8(3):437–441. doi: 10.1099/00222615-8-3-437. [DOI] [PubMed] [Google Scholar]
  12. Thorne G. M., Farrar W. E., Jr Transfer of ampicillin resistance between strains of Haemophilus influenzae type B. J Infect Dis. 1975 Sep;132(3):276–281. doi: 10.1093/infdis/132.3.276. [DOI] [PubMed] [Google Scholar]
  13. Thornsberry C., Kirven L. A. Antimicrobial susceptibility of Haemophilus influenzae. Antimicrob Agents Chemother. 1974 Nov;6(5):620–624. doi: 10.1128/aac.6.5.620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Weiser R., Asscher A. W., Wimpenny J. In vitro reversal of antibiotic resistance by ethylenediamine tetraacetic acid. Nature. 1968 Sep 28;219(5161):1365–1366. doi: 10.1038/2191365a0. [DOI] [PubMed] [Google Scholar]
  15. Williams J. D., Kattan S., Cavanagh P. Letter: Penicillinase production by Haemophilus influenzae. Lancet. 1974 Jul 13;2(7872):103–103. doi: 10.1016/s0140-6736(74)91663-8. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES