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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Apr;73(4):1314–1318. doi: 10.1073/pnas.73.4.1314

Fluorometric assay of vasopressin and oxytocin: a general approach to the assay of peptides in tissues.

K A Gruber, S Stein, L Brink, A Radhakrishnan, S Udenfriend
PMCID: PMC430258  PMID: 1063412

Abstract

A fluorometric method for the quantitative assay of vasopressin and oxytocin in individual rat pituitaries has been developed. Acid extracts of pituitaries are freed of amino acids and polyamines by passage over a copper-Sephadex column, and the peptides fraction is then labeled by reaction with fluorescamine. The resulting peptide fluorophors are separated by chromatography on a reverse-phase bonded column. Specificity of the procedure was ascertained by several criteria, including bioassay and amino-acid analysis of the eluted peptide fluorophors. The procedure serves as a model system for the assay of tissue peptides in the picomole range.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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