Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 May;73(5):1617–1620. doi: 10.1073/pnas.73.5.1617

Stimulation of prostaglandin E synthesis in cultured human umbilical vein smooth muscle cells.

R W Alexander, M A Gimbrone Jr
PMCID: PMC430350  PMID: 1064032

Abstract

Cultured human umbilical vein smooth muscle cells secreted 1.26 +/- 0.20 mug of immunoreactive prostaglandin E (iPGE) per mg of cell protein per 24 hr (mean +/- SEM). In indomethacin, an inhibitor of prostaglandin synthesis, abolished greater than 95% of this basal iPGE production (ID50 = 1.8 nM).

Full text

PDF
1618

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aiken J. W. Effects of prostaglandin synthesis inhibitors on angiotensin tachyphylaxis in the isolated coeliac and mesenteric arteries of the rabbit. Pol J Pharmacol Pharm. 1974 Jan-Apr;26(1):217–227. [PubMed] [Google Scholar]
  2. Alexander R. W., Kent K. M., Pisano J. J., Keiser H. R., Cooper T. Regulation of postocclusive hyperemia by endogenously synthesized prostaglandins in the dog heart. J Clin Invest. 1975 Jun;55(6):1174–1181. doi: 10.1172/JCI108034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Altura B. M., Malaviya D., Reich C. F., Orkin L. R. Effects of vasoactive agents on isolated human umbilical arteries and veins. Am J Physiol. 1972 Feb;222(2):345–355. doi: 10.1152/ajplegacy.1972.222.2.345. [DOI] [PubMed] [Google Scholar]
  4. Danon A., Chang L. C., Sweetman B. J., Nies A. S., Oates J. A. Synthesis of prostaglandins by the rat renal papilla in vitro. Mechanism of stimulation by angiotensin II. Biochim Biophys Acta. 1975 Apr 18;388(1):71–83. doi: 10.1016/0005-2760(75)90063-6. [DOI] [PubMed] [Google Scholar]
  5. Ferreira S. H., Moncada S., Vane J. R. Some effects of inhibiting endogenous prostaglandin formation on the responses of the cat spleen. Br J Pharmacol. 1973 Jan;47(1):48–58. doi: 10.1111/j.1476-5381.1973.tb08157.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Flower R. J. Drugs which inhibit prostaglandin biosynthesis. Pharmacol Rev. 1974 Mar;26(1):33–67. [PubMed] [Google Scholar]
  7. Gimbrone M. A., Jr, Alexander R. W. Angiotensin II stimulation of prostaglandin production in cultured human vascular endothelium. Science. 1975 Jul 18;189(4198):219–220. doi: 10.1126/science.1138377. [DOI] [PubMed] [Google Scholar]
  8. Gimbrone M. A., Jr, Cotran R. S. Human vascular smooth muscle in culture. Growth and ultrastructure. Lab Invest. 1975 Jul;33(1):16–27. [PubMed] [Google Scholar]
  9. Gryglewski R., Vane J. R. The release of prostaglandins and rabbit aorta contracting substance (RCS) from rabbit spleen and its antagonism by anti-inflammatory drugs. Br J Pharmacol. 1972 May;45(1):37–47. doi: 10.1111/j.1476-5381.1972.tb09574.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hammarström S., Samuelsson B., Bjursell G. Prostaglandin levels in normal and transformed baby-hamster-kidney fibroblasts. Nat New Biol. 1973 May 9;243(123):50–51. [PubMed] [Google Scholar]
  11. Karim S. M., Hillier K., Devlin J. Distribution of prostaglandins E1,E2, F1-alpha and F2-alpha in some animal tissues. J Pharm Pharmacol. 1968 Oct;20(10):749–753. doi: 10.1111/j.2042-7158.1968.tb09633.x. [DOI] [PubMed] [Google Scholar]
  12. Kunze H., Vogt W. Significance of phospholipase A for prostaglandin formation. Ann N Y Acad Sci. 1971 Apr 30;180:123–125. doi: 10.1111/j.1749-6632.1971.tb53191.x. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Levine L., Hinkle P. M., Voelkel E. F., Tashjian A. H., Jr Prostaglandin production by mouse fibrosarcoma cells in culture: inhibition by indomethacin and aspirin. Biochem Biophys Res Commun. 1972 May 26;47(4):888–896. doi: 10.1016/0006-291x(72)90576-1. [DOI] [PubMed] [Google Scholar]
  15. McGiff J. C., Crowshaw K., Terragno N. A., Malik K. U., Lonigro A. J. Differential effect of noradrenaline and renal nerve stimulation on vascular resistance in the dog kidney and the release of a prostaglandin E-like substance. Clin Sci. 1972 Feb;42(2):223–233. doi: 10.1042/cs0420223. [DOI] [PubMed] [Google Scholar]
  16. McGiff J. C., Itskovitz H. D. Prostaglandins and the kidney. Circ Res. 1973 Nov;33(5):479–488. doi: 10.1161/01.res.33.5.479. [DOI] [PubMed] [Google Scholar]
  17. Messina E. J., Weiner R., Kaley G. Inhibition of bradykinin vasodilation and potentiation of norepinephrine and angiotensin vasoconstriction by inhibitors of prostaglandin synthesis in skeletal muscle of the rat. Circ Res. 1975 Oct;37(4):430–437. doi: 10.1161/01.res.37.4.430. [DOI] [PubMed] [Google Scholar]
  18. Messina E. J., Weiner R., Kaley G. Microcirculatory effects of prostaglandins E1, E2, and A1 in the rat mesentery and cremaster muscle. Microvasc Res. 1974 Jul;8(1):77–89. doi: 10.1016/0026-2862(74)90066-1. [DOI] [PubMed] [Google Scholar]
  19. Needleman P., Key S. L., Denny S. E., Isakson P. C., Marshall G. R. Mechanism and modification of bradykinin-induced coronary vasodilation. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2060–2063. doi: 10.1073/pnas.72.6.2060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Needleman P., Marshall G. R., Douglas J. R., Jr Prostaglandin release from vasculature by angiotensin II: dissociation from lipolysis. Eur J Pharmacol. 1973 Sep;23(3):316–319. doi: 10.1016/0014-2999(73)90103-9. [DOI] [PubMed] [Google Scholar]
  21. Starling M. B., Elliott R. B. The effects of prostaglandins, prostaglandin inhibitors, and oxygen on the closure of the ductus arteriosus, pulmonary arteries and umbilical vessels in vitro. Prostaglandins. 1974 Nov 10;8(3):187–203. doi: 10.1016/0090-6980(74)90042-2. [DOI] [PubMed] [Google Scholar]
  22. Terragno N. A., Terragno D. A., Pacholczyk D., McGiff J. C. Prostaglandins and the regulation of uterine blood flow in pregnancy. Nature. 1974 May 3;249(452):57–58. doi: 10.1038/249057a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES