Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 May;73(5):1730–1734. doi: 10.1073/pnas.73.5.1730

Inhibition of arachidonic acid release from cells as the biochemical action of anti-inflammatory corticosteroids.

S L Hong, L Levine
PMCID: PMC430374  PMID: 1064044

Abstract

Serum stimulates the production of prostaglandins by transformed mouse fibroblasts. Hydrocortisone (cortisol) inhibits this stimulation. The half-maximal inhibition occurs at 6x10-9 M. Studies with cells labeled with [3H]arachidonic acid in their lipids show that the stimulation by serum results in the release of arachidonic acid from the cellular lipids, mostly phospholipids. Hydrocrotisone inhibits this release but does not inhibit the production of prostaglandins from exogenously supplied arachidonic acid. This inhibition of arachidonic acid release from phospholipids may be the mechanism for the anti-inflammatory action of corticosteroids.

Full text

PDF
1734

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames G. F. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bacteriol. 1968 Mar;95(3):833–843. doi: 10.1128/jb.95.3.833-843.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Flower R., Gryglewski R., Herbaczyńska-Cedro K., Vane J. R. Effects of anti-inflammatory drugs on prostaglandin biosynthesis. Nat New Biol. 1972 Jul 26;238(82):104–106. doi: 10.1038/newbio238104a0. [DOI] [PubMed] [Google Scholar]
  3. Greaves M. W., McDonald-Gibson W. Prostaglandin biosynthesis by human skin and its inhibition by corticosteroids. Br J Pharmacol. 1972 Sep;46(1):172–175. doi: 10.1111/j.1476-5381.1972.tb06861.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gryglewski R. J., Panczenko B., Korbut R., Grodzinska L., Ocetkiewicz A. Corticosteroids inhibit prostaglandin release from perfused mesenteric blood vessels of rabbit and from perfused lungs of sensitized guinea pig. Prostaglandins. 1975 Aug;10(2):343–355. doi: 10.1016/0090-6980(75)90053-2. [DOI] [PubMed] [Google Scholar]
  5. Hong S. L., Polsky-Cynkin R., Levine L. Stimulation of prostaglandin biosynthesis by vasoactive substances in methylcholanthrene-transformed mouse BALB/3T3. J Biol Chem. 1976 Feb 10;251(3):776–780. [PubMed] [Google Scholar]
  6. Kantrowitz F., Robinson D. R., McGuire M. B., Levine L. Corticosteroids inhibit prostaglandin production by rheumatiod synovia. Nature. 1975 Dec 25;258(5537):737–739. doi: 10.1038/258737a0. [DOI] [PubMed] [Google Scholar]
  7. Lands W. E., Samuelsson B. Phospholipid precursors of prostaglandins. Biochim Biophys Acta. 1968 Oct 22;164(2):426–429. doi: 10.1016/0005-2760(68)90168-9. [DOI] [PubMed] [Google Scholar]
  8. Lewis D. A., Symons A. M., Ancill R. J. Action of anti-inflammatory steroids on the lytic action of phospholipase C and 2,4,6-trinitrobenzene sulphonic acid on lysosomes. Biochem Pharmacol. 1974 Jan 15;23(2):467–470. doi: 10.1016/0006-2952(74)90441-9. [DOI] [PubMed] [Google Scholar]
  9. Lewis G. P., Piper P. J. Inhibition of release of prostaglandins as an explanation of some of the actions of anti-inflammatory corticosteroids. Nature. 1975 Mar 27;254(5498):308–311. doi: 10.1038/254308a0. [DOI] [PubMed] [Google Scholar]
  10. Lieber M. M., Livingston D. M., Todaro G. J. Superinduction of endogenous type C virus by 5-bromodeoxyuridine from transformed mouse clones. Science. 1973 Aug 3;181(4098):443–444. doi: 10.1126/science.181.4098.443. [DOI] [PubMed] [Google Scholar]
  11. Persellin R. H., Ku L. C. Effects of steroid hormones on human polymorphonuclear leukocyte lysosomes. J Clin Invest. 1974 Oct;54(4):919–925. doi: 10.1172/JCI107832. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ramwell P. W., Shaw J. E. Biological significance of the prostaglandins. Recent Prog Horm Res. 1970;26:139–187. doi: 10.1016/b978-0-12-571126-5.50008-x. [DOI] [PubMed] [Google Scholar]
  13. Tashjian A. H., Jr, Voelkel E. F., McDonough J., Levine L. Hydrocortisone inhibits prostaglandin production by mouse fibrosarcoma cells. Nature. 1975 Dec 25;258(5537):739–741. doi: 10.1038/258739a0. [DOI] [PubMed] [Google Scholar]
  14. Vane J. R. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat New Biol. 1971 Jun 23;231(25):232–235. doi: 10.1038/newbio231232a0. [DOI] [PubMed] [Google Scholar]
  15. WEISSMANN G., THOMAS L. Studies on lysosomes. II. The effect of cortisone on the release of acid hydrolases from a large granule fraction of rabbit liver induced by an excess of vitamin A. J Clin Invest. 1963 May;42:661–669. doi: 10.1172/JCI104757. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES