Abstract
Protoplasts of two doubly auxotrophis strains of Bacillus megaterium were fused by nascent calcium phosphate or by polyethylene glycol treatment, and then allowed to revert to bacillary form on selection media. The colonies formed were analyzed and found to be mixed populations of individual bacteria apparently containing parental, recombinant, and segregating genotypes. The evidence suggested that segregation was transitory, lasting only for a few clonings and reisolations.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ferenczy L., Kevei F., Zsolt J. Fusion of fungal protoplasts. Nature. 1974 Apr 26;248(5451):793–794. doi: 10.1038/248793a0. [DOI] [PubMed] [Google Scholar]
- Fodor K., Hadlaczky G., Alföldi L. Reversion of Bacillus megaterium protoplasts to the bacillary form. J Bacteriol. 1975 Jan;121(1):390–391. doi: 10.1128/jb.121.1.390-391.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HARRIS H., WATKINS J. F. HYBRID CELLS DERIVED FROM MOUSE AND MAN: ARTIFICIAL HETEROKARYONS OF MAMMALIAN CELLS FROM DIFFERENT SPECIES. Nature. 1965 Feb 13;205:640–646. doi: 10.1038/205640a0. [DOI] [PubMed] [Google Scholar]
- HOTCHKISS R. D. Transfer of penicillin resistance in pneumococci by the desoxyribonucleate derived from resistant cultures. Cold Spring Harb Symp Quant Biol. 1951;16:457–461. doi: 10.1101/sqb.1951.016.01.032. [DOI] [PubMed] [Google Scholar]
- Keller W. A., Melchers G. The effect of high pH and calcium on tobacco leaf protoplast fusion. Z Naturforsch C. 1973 Nov-Dec;28(11):737–741. doi: 10.1515/znc-1973-11-1215. [DOI] [PubMed] [Google Scholar]
- Power J. B., Cummins S. E., Cocking E. C. Fusion of isolated plant protoplasts. Nature. 1970 Mar 14;225(5237):1016–1018. doi: 10.1038/2251016a0. [DOI] [PubMed] [Google Scholar]
- Schaeffer P., Cami B., Hotchkiss R. D. Fusion of bacterial protoplasts. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2151–2155. doi: 10.1073/pnas.73.6.2151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEIBULL C. The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme. J Bacteriol. 1953 Dec;66(6):688–695. doi: 10.1128/jb.66.6.688-695.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]