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. 1977 Mar;74(3):994–998. doi: 10.1073/pnas.74.3.994

Rous sarcoma virus genome is terminally redundant: the 3' sequence.

D E Schwartz, P C Zamecnik, H L Weith
PMCID: PMC430560  PMID: 66684

Abstract

A sequence of 20 nucleotide residues immediately adjacent to the 3'-terminal poly(A) in Rous sarcoma virus (Prague strain, subgroup C) 35S RNA has been determined by extension of a riboguanylic acid-terminated oligothymidylic acid primer hybridized at the 5' end of the 3'-terminal poly(A) with purified reverse transcriptase (RNA-directed DNA polymerase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) from avian myeloblastosis virus. The sequence is 5'GCCAUUUUACCAUUCACCACpoly(A)3'. This same nucleotide sequence, excluding the poly(A) segment, has also been found at the 5' terminus of Rous sarcoma virus RNA (W. A. Haseltine, A. Maxam, and W. Gilbert, this issue pp. 989-993), and therefore the RNA genome of this virus is terminally redundant. Possible mechanisms for endogenous in vitro copying of the complete RNA genome by reverse transcriptase which involve terminally repeated nucleotide sequences are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cheng C. C., Brownlee G. G., Carey N. H., Doel M. T., Gillam S., Smith M. The 3' terminal sequence of chicken ovalbumin messenger RNA and its comparison with other messenger RNA molecules. J Mol Biol. 1976 Nov 15;107(4):527–547. doi: 10.1016/s0022-2836(76)80081-2. [DOI] [PubMed] [Google Scholar]
  2. Collett M. S., Faras A. J. Evidence for circularization of the avian oncornavirus RNA genome during proviral DNA synthesis from studies of reverse transcription in vitro. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1329–1332. doi: 10.1073/pnas.73.4.1329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Donelson J. E., Barrell B. G., Weith H. L. The use of primed synthesis by DNA polymerase I to study an intercistronic sequence of phiX-174 DNA. Eur J Biochem. 1975 Oct 15;58(2):383–395. doi: 10.1111/j.1432-1033.1975.tb02385.x. [DOI] [PubMed] [Google Scholar]
  4. Faras A. J., Dahlberg J. E., Sawyer R. C., Harada F., Taylor J. M., Levinson W. E., Bishop J. M., Goodman H. M. Transcription of DNA from the 70S RNA of Rous sarcoma virus. II. Structure of a 4S RNA primer. J Virol. 1974 May;13(5):1134–1142. doi: 10.1128/jvi.13.5.1134-1142.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guntaka R. V., Richards O. C., Shank P. R., Kung H. J., Davidson N. Covalently closed circular DNA of avian sarcoma virus: purification from nuclei of infected quail tumor cells and measurement by electron microscopy and gel electrophoresis. J Mol Biol. 1976 Sep 15;106(2):337–357. doi: 10.1016/0022-2836(76)90090-5. [DOI] [PubMed] [Google Scholar]
  6. Haseltine W. A., Kleid D. G., Panet A., Rothenberg E., Baltimore D. Ordered transcription of RNA tumor virus genomes. J Mol Biol. 1976 Sep 5;106(1):109–131. doi: 10.1016/0022-2836(76)90303-x. [DOI] [PubMed] [Google Scholar]
  7. Haseltine W. A., Maxam A. M., Gilbert W. Rous sarcoma virus genome is terminally redundant: the 5' sequence. Proc Natl Acad Sci U S A. 1977 Mar;74(3):989–993. doi: 10.1073/pnas.74.3.989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Proudfoot N. J., Brownlee G. G. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. doi: 10.1038/263211a0. [DOI] [PubMed] [Google Scholar]
  9. Roychoudhury R., Kössel H. Synthetic polynucleotides. Enzymic synthesis of ribonucleotide terminated oligodeoxynucleotides and their use as primers for the enzymic synthesis of polydeoxynucleotides. Eur J Biochem. 1971 Oct 14;22(3):310–320. doi: 10.1111/j.1432-1033.1971.tb01546.x. [DOI] [PubMed] [Google Scholar]
  10. Stephenson M. L., Wirthlin L. S., Scott J. F., Zamecnik P. C. The 3'-terminal nucleosides of the high molecular weight RNA of avian myeloblastosis virus. Proc Natl Acad Sci U S A. 1972 May;69(5):1176–1180. doi: 10.1073/pnas.69.5.1176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. TOMLINSON R. V., TENER G. M. THE EFFECT OF UREA, FORMAMIDE, AND GLYCOLS ON THE SECONDARY BINDING FORCES IN THE ION-EXCHANGE CHROMATOGRAPHY OF POLYNUCLEOTIDES OF DEAE-CELLULOSE. Biochemistry. 1963 Jul-Aug;2:697–702. doi: 10.1021/bi00904a013. [DOI] [PubMed] [Google Scholar]
  12. Taylor J. M., Illmensee R. Site on the RNA of an avian sarcoma virus at which primer is bound. J Virol. 1975 Sep;16(3):553–558. doi: 10.1128/jvi.16.3.553-558.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Temin H. M. The DNA provirus hypothesis. Science. 1976 Jun 11;192(4244):1075–1080. doi: 10.1126/science.58444. [DOI] [PubMed] [Google Scholar]

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