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. 1977 Mar;74(3):1172–1176. doi: 10.1073/pnas.74.3.1172

Proposed involvement of an internal promoter in regulation and synthesis of mitochondrial and cytoplasmic leucyl-tRNA synthetases of Neurospora.

P M Beauchamp, E W Horn, S R Gross
PMCID: PMC430637  PMID: 139609

Abstract

Genetic analysis of an electrophoretic variant of the mitochondrial leucyl-tRNA synthetase [L-leucine:tRNALeu ligase (AMP-forming), EC 6.1.1.4] indicates that it is either an allele of or linked closely to leu-5ts, a mutant that is known to produce a cytoplasmic leucyl-tRNA synthetase with an altered affinity for leucine as well as being deficient in the production of the mitochondrial enzyme. Immunological analysis indicates that the two synthetases have little, if any, structural homology. The pattern of synthesis of the enzymes in leu-5ts revertants, the reciprocal relationship of the production of the two enzymes in response to a negative regulatory element, presumably of mitochondrial origin, as well as the lack of detectable structural homology, led to the proposal that the phenotype of leu-5ts results from a mutational alteration in the structural gene for the cytoplasmic enzyme in a region involved in the initiation of transcription of the adjacent gene for the mitochondrial enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barath Z., Küntzel H. Cooperation of mitochondrial and nuclear genes specifying the mitochondrial genetic apparatus in Neurospora crassa. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1371–1374. doi: 10.1073/pnas.69.6.1371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barrell B. G., Air G. M., Hutchison C. A., 3rd Overlapping genes in bacteriophage phiX174. Nature. 1976 Nov 4;264(5581):34–41. doi: 10.1038/264034a0. [DOI] [PubMed] [Google Scholar]
  3. Beuchamp P. M., Gross S. R. Increased mitochondrial leucyl- and phenylalanyl-tRNA synthetase activity as a result of inhibition of mitochondrial protein synthesis. Nature. 1976 May 27;261(5558):338–340. doi: 10.1038/261338a0. [DOI] [PubMed] [Google Scholar]
  4. Chiu A. O., Suyama Y. Immunologic studies on intracellular isoenzymes; the mitochondrial and cytoplasmic leucyl-tRNA synthetases. Biochim Biophys Acta. 1973 Apr 11;299(4):557–563. doi: 10.1016/0005-2787(73)90227-x. [DOI] [PubMed] [Google Scholar]
  5. Cuatrecasas P. Insulin-sepharose: immunoreactivity and use in the purification of antibody. Biochem Biophys Res Commun. 1969 May 22;35(4):531–537. doi: 10.1016/0006-291x(69)90379-9. [DOI] [PubMed] [Google Scholar]
  6. Gross S. R., McCoy M. T., Gilmore E. B. Evidence for the involvement of a nuclear gene in the productin of the mitochondrial leucyl-tRNA synthetase of Neurospora. Proc Natl Acad Sci U S A. 1968 Sep;61(1):253–260. doi: 10.1073/pnas.61.1.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jackson E. N., Yanofsky C. Internal promoter of the tryptophan operon of Escherichia coli is located in a structural gene. J Mol Biol. 1972 Aug 21;69(2):307–313. doi: 10.1016/0022-2836(72)90232-x. [DOI] [PubMed] [Google Scholar]
  8. LESTER H. E., GROSS S. R. Efficient method for selection of auxotrophic mutants of Neurospora. Science. 1959 Feb 27;129(3348):572–572. doi: 10.1126/science.129.3348.572. [DOI] [PubMed] [Google Scholar]
  9. Lewis C. M., Holliday R. Mistranslation and ageing in Neurospora. Nature. 1970 Nov 28;228(5274):877–880. doi: 10.1038/228877a0. [DOI] [PubMed] [Google Scholar]
  10. Printz D. B., Gross S. R. An apparent relationship between mistranslation and an altered leucyl-tRNA synthetase in a conditional lethal mutant of Neurospora crassa. Genetics. 1967 Mar;55(3):451–467. doi: 10.1093/genetics/55.3.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rosenberg E., Mora C., Edwards D. L. Selection of extranuclear mutants of Neurospora crassa. Genetics. 1976 May;83(1):11–24. doi: 10.1093/genetics/83.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Strickland W N, Perkins D D, Veatch C C. Linkage Data for Group V Markers in Neurospora. Genetics. 1959 Nov;44(6):1221–1226. doi: 10.1093/genetics/44.6.1221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Weeks C. O., Gross S. R. Mutation and "reversion" at the leu-5 locus of neurospora and its effect on the cytoplasmic and mitochondrial leucyl-tRNA synthetases. Biochem Genet. 1971 Dec;5(6):505–516. doi: 10.1007/BF00485668. [DOI] [PubMed] [Google Scholar]

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